Flow-dependent entrapment of large bioparticles in porous process media.
Flow-dependent entrapment of large bioparticles in porous process media.
复制标题
DOI：
10.1002/bit.22370
复制
发表时间：
2009-09-01
影响因子：
3.8
通讯作者：
Lenhoff, Abraham M.
中科院分区：
文献类型：
--
作者：
Trilisky, Egor I.;Lenhoff, Abraham M.
关键词：
对生物分子纯化的需求也延伸到更大的生物颗粒。例如，许多疫苗和基因递送载体的生产需要病毒纯化，并且理解多孔介质中的病毒去除在治疗性蛋白质的下游加工和土壤中水的纯化中也是重要的。对流截留机制保留大的生物颗粒在这里讨论的基础上保留大的生物颗粒在孔收缩在足够高的流速，即使在非约束条件下。一个简单的方程来预测是否预期会发生这样的截留在一个给定的系统是基于一个Péclet数，这是成比例的流速和生物颗粒直径的立方。为了验证这一理论，将腺病毒掺入色谱床上。正如理论所预期的那样，在非相互作用条件下，随着流速的增加，逐渐大量的病毒被捕获。截留是可逆的流量减少后，这在所提出的模型，是基于从孔隙收缩扩散逃逸的可能性。这种机制可用于病毒纯化或去除，该理论还解释了整体柱和膜比传统色谱床更难清洁的轶事证据，特别是在高流速下。
The need for purification of biomolecules extends to larger bioparticles as well. For example, virus purification is required for production of many vaccines and gene delivery vectors, and understanding virus removal in porous media is also important in downstream processing of therapeutic proteins and in purification of water in soils. A convective entrapment mechanism for retention of large bioparticles is discussed here based on retention of large bioparticles in pore constrictions at high enough flow rates, even under non-binding conditions. A simple equation to predict whether such entrapment is expected to occur in a given system is derived based on a Péclet number that is proportional to the flow rate and to the cube of the bioparticle diameter. To test the theory, adenovirus was spiked onto chromatographic beds. As expected from the theory, under non-interacting conditions a progressively larger amount of virus becomes trapped with increasing flow rate. The entrapment is reversible upon flow rate reduction, which, within the proposed model, is based on the possibility of diffusive escape from pore constrictions. This mechanism can be exploited for virus purification or removal, and the theory also explains the anecdotal evidence that monoliths and membranes are more difficult to clean than conventional chromatographic beds, especially at high flow rates.
登录
查看更多内容
影响因子：
11.4
作者：
Fabry, CMS;Rosa-Calatrava, M;Schoehn, G
通讯作者：
Schoehn, G
影响因子：
4.1
作者：
Arvidsson, P;Plieva, FM;Mattiasson, B
通讯作者：
Mattiasson, B
影响因子：
7.4
作者：
Giovannini, R;Freitag, R;Tennikova, TB
通讯作者：
Tennikova, TB
DOI：
10.1016/0304-4165(76)90026-x
发表时间：
1976-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
OLIVER, CJ;SHORTRIDGE, KF;BELYAVIN, G
通讯作者：
BELYAVIN, G
DOI：
10.1016/j.jchromb.2004.01.050
发表时间：
2004-07-25
影响因子：
3
作者：
Plieva, FM;Savina, IN;Mattiasson, B
通讯作者：
Mattiasson, B