Acyl Carrier Protein 3 Is Involved in Oxidative Stress Response in Pseudomonas aeruginosa.
Acyl Carrier Protein 3 Is Involved in Oxidative Stress Response in Pseudomonas aeruginosa.
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DOI：
10.3389/fmicb.2018.02244
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发表时间：
2018
影响因子：
5.2
通讯作者：
Davies C
中科院分区：
文献类型：
--
作者：
Chen W;Wang B;Gruber JD;Zhang YM;Davies C
人类机会致病菌铜绿假单胞菌表达三种酰基载体蛋白（ACP）：AcpP，Acp1和Acp3。AcpP在膜脂肪酸合成（FAS）中的功能近年来得到证实，但Acp1和Acp3的生理功能尚不清楚。为了解决这个问题，我们研究了Acp3在铜绿假单胞菌中的生理作用。我们发现Acp3的表达在细胞生长的对数期显著增加，并且其转录受QS调节因子LasR和RhlR的控制。从铜绿假单胞菌菌株PAO 1中缺失acp 3导致较厚的生物膜形成，菌株对过氧化氢的抗性增加，以及在小鼠感染模型中的更高持久性。串联亲和纯化（TAP）实验揭示了Acp3的几种新的蛋白结合伴侣，包括KatA，铜绿假单胞菌中的主要过氧化氢酶。发现Acp 3抑制KatA的过氧化氢酶活性，并且与Acp 3的抑制作用一致，acp 3缺失菌株中存在较少的活性氧物质。总体而言，我们的研究表明，ACP3具有与经典ACPP不同的功能，并可能参与氧化应激反应。
The human opportunistic pathogen Pseudomonas aeruginosa expresses three acyl carrier proteins (ACPs): AcpP, Acp1, and Acp3. The function of AcpP in membrane fatty acid synthesis (FAS) was confirmed recently, but the physiological roles of Acp1 and Acp3 remain unclear. To address this, we investigated the physiological role of Acp3 in P. aeruginosa. We found that expression of Acp3 dramatically increases in the log phase of cell growth and that its transcription is under the control of the QS regulators LasR and RhlR. Deletion of acp3 from P. aeruginosa strain PAO1 results in thicker biofilm formation, increased resistance of the strain to hydrogen peroxide, and higher persistence in a mouse infection model. Tandem affinity purification (TAP) experiments revealed several novel protein-binding partners of Acp3, including KatA, the major catalase in P. aeruginosa. Acp3 was found to repress the catalase activity of KatA and, consistent with inhibition by Acp3, less reactive oxygen species are present in the acp3 deletion strain. Overall, our study reveals that Acp3 has a distinct function from that of the canonical AcpP and may be involved in the oxidative stress response.
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影响因子：
--
作者：
Dulcey CE;Dekimpe V;Fauvelle DA;Milot S;Groleau MC;Doucet N;Rahme LG;Lépine F;Déziel E
通讯作者：
Déziel E
DOI：
10.1073/pnas.1702849114
发表时间：
2017-07-03
影响因子：
11.1
作者：
Cory, Seth A.;Van Vranken, Jonathan G.;Barondeau, David P.
通讯作者：
Barondeau, David P.
影响因子：
4.8
作者：
De lay, Nicholas R.;Cronan, John E.
通讯作者：
Cronan, John E.
影响因子：
3.1
作者：
HASSETT, DJ;CHARNIGA, L;COHEN, MS
通讯作者：
COHEN, MS
影响因子：
3.7
作者：
Angelini S;My L;Bouveret E
通讯作者：
Bouveret E