CDNA cloning of p112, the largest regulatory subunit of the human 26s proteasome, and functional analysis of its yeast homologue, sen3p.
CDNA cloning of p112, the largest regulatory subunit of the human 26s proteasome, and functional analysis of its yeast homologue, sen3p.
复制标题
p112（人类 26s 蛋白酶体最大的调节亚基）的 cDNA 克隆，及其酵母同源物 sen3p 的功能分析。
DOI：
10.1091/mbc.7.6.853
复制
发表时间：
1996
影响因子：
3.3
通讯作者：
Tanaka,K
中科院分区：
文献类型：
--
作者：
Yokota,K;Kagawa,S;Shimizu,Y;Akioka,H;Tsurumi,C;Noda,C;Fujimuro,M;Yokosawa,H;Fujiwara,T;Takahashi,E;Ohba,M;Yamasaki,M;DeMartino,GN;Slaughter,CA;Toh-e,A;Tanaka,K
The 26S proteasome is a large multisubunit protease complex, the largest regulatory subunit of which is a component named p112. Molecular cloning of cDNA encoding human p112 revealed a polypeptide predicted to have 953 amino acid residues and a molecular mass of 105,865. The human p112 gene was mapped to the q37.1-q37.2 region of chromosome 2. Computer analysis showed that p112 has strong similarity to the Saccharomyces cerevisiae Sen3p, which has been listed in a gene bank as a factor affecting tRNA splicing endonuclease. The SEN3 also was identified in a synthetic lethal screen with the nin1-1 mutant, a temperature-sensitive mutant of NIN1. NIN1 encodes p31, another regulatory subunit of the 26S proteasome, which is necessary for activation of Cdc28p kinase. Disruption of the SEN3 did not affect cell viability, but led to temperature-sensitive growth. The human p112 cDNA suppressed the growth defect at high temperature in a SEN3 disruptant, indicating that p112 is a functional homologue of the yeast Sen3p. Maintenance of SEN3 disruptant cells at the restrictive temperature resulted in a variety of cellular dysfunctions, including defects in proteolysis mediated by the ubiquitin pathway, in the N-end rule system, in the stress response upon cadmium exposure, and in nuclear protein transportation. The functional abnormality induced by SEN3 disruption differs considerably from various phenotypes shown by the nin1-1 mutation, suggesting that these two regulatory subunits of the 26S proteasome play distinct roles in the various processes mediated by the 26S proteasome.
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DOI：
--
发表时间：
1994-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Realini;W. Dubiel;G. Pratt;K. Ferrell;M. Rechsteiner
通讯作者：
C. Realini;W. Dubiel;G. Pratt;K. Ferrell;M. Rechsteiner
影响因子：
64.8
作者：
KALDERON, D;RICHARDSON, WD;SMITH, AE
通讯作者：
SMITH, AE
拟南芥 MBP1 基因编码 26S 蛋白酶体的保守泛素识别组件。
DOI：
--
发表时间：
1996
影响因子：
11.1
作者：
S. Nocker;Quinn Deveraux;Martin Rechsteiner;R. Vierstra
通讯作者：
R. Vierstra
泛素 C 末端水解酶活性与 26 S 蛋白酶复合物相关。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Eytan,E;Armon,T;Heller,H;Beck,S;Hershko,A
通讯作者：
Hershko,A
Mov 34 突变的分子分析：小鼠体内因原病毒整合而破坏的转录本在果蝇中是保守的。
DOI：
10.1242/dev.109.1.235
发表时间：
1990
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Gridley,T;Gray,DA;Orr-Weaver,T;Soriano,P;Barton,DE;Francke,U;Jaenisch,R
通讯作者：
Jaenisch,R