A tissue factor-cascade-targeted nanoparticle forsite-directed inducing thrombosis
A tissue factor-cascade-targeted nanoparticle forsite-directed inducing thrombosis
复制标题
组织因子级联靶向纳米颗粒用于定向诱导血栓形成
DOI：
10.1177/0885328217722740
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发表时间：
2017-07
影响因子：
2.9
通讯作者：
Yu Hu
中科院分区：
文献类型：
--
作者：
Weiwei Jin;Yanxue Yin;Bo Zhang;Heng Mei;Huafang Wang;Tao Guo;Wei Shi;Yu Hu
组织因子是级联反应的上游成分，在病理条件下是高表达的因子。在这项研究中，组织因子级联靶向诱导局部血栓形成的策略，结合ENP-HMME和光化学。体外研究表明，EGFP-EGF 1蛋白偶联纳米粒可显著促进组织因子过表达脑毛细血管内皮细胞对纳米粒的摄取。活体脑三维成像和斑点图显示，组织因子级联靶向策略成功诱导了预期位置的血栓形成。体内多光谱荧光成像显示，当ENP-HMME与光化学结合时，观察到在梗死半球中的较高积聚，这可能表明光化学诱导的组织因子级联比负载HMME的纳米颗粒（NP-HMME）招募更多ENP-HMME。提示组织因子级联靶向策略具有诱导局部血栓形成的潜力，可应用于多种高血管性疾病的治疗。
Tissue factor is an upstream component of the cascade and a high-expressing factor under phathological conditions. In this study, a tissue factor cascade-targeted strategy for inducing local thrombosis was developed by combining ENP-HMME and photochemistry. In vitro study showed that protein EGFP-EGF1 conjugation to the nanoparticles could significantly contribute to the uptake of nanoparticles by tissue factor over-expressed brain capillary endothelial cells. Three-dimensional imaging and specklegram of brains in vivo showed that tissue factor cascade-targeted strategy successfully induced thrombosis of expected position. As shown by the in vivo multispectral fluorescent imaging, when ENP-HMME was combined with photochemistry, higher accumulation in the infarction hemisphere was observed, which might suggest that the photochemistry inducing tissue factor cascade recruited more ENP-HMME than HMME-loaded nanoparticles (NP-HMME). The data indicated the tissue factor cascade-targeted strategy has potential to induce local thrombosis, and might be applied in the treatment of a variety of hypervascular diseases.
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