Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy.
Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy.
复制标题
通过块面扫描电子显微镜揭示的足细胞的三维结构。
DOI：
10.1038/srep08993
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发表时间：
2015-03-11
影响因子：
4.6
通讯作者：
Sakai T
中科院分区：
文献类型：
--
作者：
Ichimura K;Miyazaki N;Sadayama S;Murata K;Koike M;Nakamura K;Ohta K;Sakai T
块面成像是一种扫描电子显微镜技术，其能够更容易地直接从树脂包埋的生物样品的表面获得具有与透射电子显微照片类似的质量的系列超微结构图像。在本研究中，我们分析了足细胞的三维结构，使用系列块面成像。以前认为足细胞分为三种亚细胞区室：细胞体，初级突起和足突，它们简单地按此顺序排列。当从基底面观察重建的足细胞时，足突从初级突的基底面上形成的脊状突起分支出来，其结构与通常的足突相似。此外，从细胞体中，足突也通过脊状突起出现，如在初级突起中所发现的。嵴状突起将细胞体和初级突固定在肾小球基底膜上，并将足突与细胞体和初级突连接起来。总之，连续块面成像是一个强大的工具，通过其能力，揭示新的结构，难以确定传统的透射和扫描电子显微镜单独的足细胞的三维结构的清晰理解。
Block-face imaging is a scanning electron microscopic technique which enables easier acquisition of serial ultrastructural images directly from the surface of resin-embedded biological samples with a similar quality to transmission electron micrographs. In the present study, we analyzed the three-dimensional architecture of podocytes using serial block-face imaging. It was previously believed that podocytes are divided into three kinds of subcellular compartment: cell body, primary process, and foot process, which are simply aligned in this order. When the reconstructed podocytes were viewed from their basal side, the foot processes were branched from a ridge-like prominence, which was formed on the basal surface of the primary process and was similar to the usual foot processes in structure. Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process. The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process. In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.
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影响因子：
3.5
作者：
Sonomura T;Furuta T;Nakatani I;Yamamoto Y;Unzai T;Matsuda W;Iwai H;Yamanaka A;Uemura M;Kaneko T
通讯作者：
Kaneko T
影响因子：
4.6
作者：
Kubota, Yoshiyuki;Karube, Fuyuki;Nomura, Masaki;Gulledge, Allan T.;Mochizuki, Atsushi;Schertel, Andreas;Kawaguchi, Yasuo
通讯作者：
Kawaguchi, Yasuo
影响因子：
19.6
作者：
Inokuchi, S;Shirato, I;Sakai, T
通讯作者：
Sakai, T
影响因子：
3
作者：
Heymann, Jurgen A. W.;Hayles, Mike;Subramaniam, Sriram
通讯作者：
Subramaniam, Sriram
影响因子：
3.6
作者：
Ichimura, Koichiro;Kurihara, Hidetake;Sakai, Tatsuo
通讯作者：
Sakai, Tatsuo