Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma.
Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma.
复制标题
沙利度胺影响黑色素瘤的生长和血管生成拟态通道的形成
DOI：
10.1186/1756-9966-27-60
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发表时间：
2008-11-04
期刊：
影响因子：
--
通讯作者：
Sun B
中科院分区：
文献类型：
--
作者：
Zhang S;Li M;Gu Y;Liu Z;Xu S;Cui Y;Sun B
目的观察沙利度胺对C57小鼠黑色素瘤生长及血供的影响。从接种后第5天开始，对治疗组以200 mg/kg的剂量每天给予1次沙利度胺，对照组同样给予等体积的0.5%羧甲基纤维素。接种后每天测量肿瘤的直径，直到第19天处死小鼠。采用免疫组化和PAS组织化学双重染色，计数不同的血供类型。结果治疗组肿瘤体积（P = 0.019）、血管生成拟态（P = 0.03）和马赛克血管（P = 0.004）均较对照组明显缩小，肿瘤组织中VEGF、NF-κ B、PCNA、MMP-2和MMP-9的表达均较对照组明显减少。VEGF（P = 0.004）、NF-κ B B（P = 0.009），PCNA（P = 0.002），MMP-2（P = 0.000），MMP-9（P = 0.002）蛋白表达和MMP-2（P = 0.000）和MMP-9（P = 0.000）结论沙利度胺能抑制血管生成拟态通道和马赛克血管的形成，抑制血管生成拟态通道和马赛克血管的形成。通过调节血管生成因子对黑色素瘤的作用，并可诱导黑色素瘤细胞坏死，这可能与NF-κ B信号通路有关。
AimsTo observe the effects of thalidomide on melanoma tumor growth and blood supply patterns in C57 mice.MethodsThirty mice inoculated subcutaneously with B16F10 cells were randomly divided into the treatment group and the control group. Thalidomide was administered once a day at a dose of 200 mg/kg for the treatment group starting on the fifth day after inoculation, and an equivalent volume of 0.5% carboxylmethyl cellulose was administered similarly in the control group. The diameter of the tumors was measured daily after inoculation until the mice were sacrificed on the 19th day. The different blood supply patterns were counted after immunohistochemical and PAS histochemical double-Staining. VEGF, NF-κB, PCNA, MMP-2 and MMP-9 expression in tumor tissue was also assessed.ResultsThe tumor volume(P= 0.019) and the number of vasculogenic mimicry(P= 0.03) and mosaic vessels(P= 0.004) in the treatment group were significantly decreased compared with the control group. VEGF(P= 0.004), NF-κB(P= 0.009), PCNA(P= 0.002), MMP-2 (P= 0.000), MMP-9(P= 0.002) protein expression and MMP-2(P= 0.000) and MMP-9(P= 0.000) mRNA in the treatment group were significantly lower than those in the control groups.ConclusionThalidomide inhibits vasculogenic mimicry channel and mosaic vessels formation in melanoma through the regulation of vasculogenic factors, and it can induce necrosis of melanoma cells, which may be related with the NF-κB signaling pathway.
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