Human mesenchymal stem cells in spheroids improve fertility in model animals with damaged endometrium.
Human mesenchymal stem cells in spheroids improve fertility in model animals with damaged endometrium.
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DOI：
10.1186/s13287-018-0801-9
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发表时间：
2018-02-26
影响因子：
7.5
通讯作者：
Nikolsky N
中科院分区：
文献类型：
--
作者：
Domnina A;Novikova P;Obidina J;Fridlyanskaya I;Alekseenko L;Kozhukharova I;Lyublinskaya O;Zenin V;Nikolsky N
Asherman综合征(AS)是由子宫内膜破坏引起的妇科疾病之一。对于一些AS病例，可用的手术方法和激素治疗无效。干细胞移植可能为AS的治疗提供一种潜在的替代方案。将排列成球状的人子宫内膜间充质干细胞(EMSC)移植到AS损伤的大鼠体内。治疗反应定义为妊娠结局和产仔数。球体内应用EMSC可显著提高AS模型大鼠的生育力。球状组织的EMSC保留了单层EMSC的所有特性：生长特性、CD标志物的表达和分化潜能。在组装成球体的EMSC中，血管生成因子和抗炎因子的合成显著增加。人子宫内膜间充质干细胞(EMSC)可成功应用于Asherman综合征(AS)大鼠模型的治疗。球状的EMSC比单层的EMSC具有更好的治疗效果。在球体内移植EMSC后，AS大鼠的妊娠结局和产仔数高于接受自体大鼠骨髓细胞移植的大鼠。这表明异体EMSC在不使用自体细胞的情况下具有治疗的可能性。
Asherman’s syndrome (AS) is one of the gynecological disorders caused by the destruction of the endometrium. For some cases of AS available surgical methods and hormonal therapy are ineffective. Stem cell transplantation may offer a potential alternative for AS cure. Human endometrial mesenchymal stem cells (eMSC) organized in spheroids were transplanted in rats with damaged endometrium modeled on AS. Treatment response was defined as pregnancy outcome and litter size. Application of eMSC in spheroids significantly improved the rat fertility with the AS model. eMSC organized in spheroids retain all properties of eMSC in monolayer: growth characteristics, expression of CD markers, and differentiation potential. Synthesis of angiogenic and anti-inflammatory factors drastically increased in eMSC assembled into spheroids. Human endometrial mesenchymal stem cells (eMSC) can be successfully applied for Asherman’s syndrome (AS) treatment in the rat model. eMSC organized in spheroids were more therapeutically effective than the cells in monolayer. After transplantation of eMSC in spheroids the pregnancy outcome and litter size in rats with AS was higher than in rats that received autologous rat bone marrow cells. It suggests the therapeutic plausibility of heterologous eMSC in case of failure to use autologous cells.
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影响因子：
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DOI：
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