MR and optical imaging of early micrometastases in lymph nodes: triple labeling with nano-sized agents yielding distinct signals.
MR and optical imaging of early micrometastases in lymph nodes: triple labeling with nano-sized agents yielding distinct signals.
复制标题
淋巴结早期微型转移酶的MR和光学成像：具有纳米尺寸剂的三重标记，产生不同的信号。
DOI：
10.1002/cmmi.489
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发表时间：
2012-03
影响因子：
--
通讯作者：
Kobayashi H
中科院分区：
文献类型：
--
作者：
Kosaka N;Bernardo M;Mitsunaga M;Choyke PL;Kobayashi H
很少有成像方法可用于描绘淋巴系统内的体内癌细胞迁移。检测这种早期微转移需要极高的靶向背景。在这项研究中，我们用氧化铁（SPIO）和量子点（QD）的小颗粒双标记人乳腺癌细胞（MDA-MB 468），并使用体内MRI和光学成像在小鼠淋巴系统中追踪这些细胞。第6代钆树枝状聚合物为基础的MRI造影剂（Gd-G6）用于可视化区域淋巴管和淋巴结。由于Gd-G6缩短了T1，导致高信号，而SPIO标记的癌细胞大大降低了信号，因此在引流淋巴盆内同时可见少量细胞。在成像前24小时，将一百万个双标记的癌细胞皮下注射到小鼠的爪中。分别在皮内注射Gd-G6前后，用3D-T1 w-FFE和balanced-FFE序列采集全身图像，进行癌细胞追踪和MR淋巴管造影。体内MRI清楚地显示了使用引流淋巴管从爪迁移到腋窝淋巴结的标记的癌细胞。使用荧光手术显微镜的体内光学成像显示了腋窝淋巴结中具有高空间分辨率的微小癌细胞簇。因此，使用MRI和光学成像的组合，可以描绘淋巴系统内的宏观和早期微转移。该平台为研究和治疗动物模型中的淋巴转移提供了多功能的研究工具。
Few imaging methods are available for depicting in vivo cancer cell migration within the lymphatic system. Detection of such early micrometastases requires extremely high target to background. In this study, we dual-labeled human breast cancer cells (MDA-MB468) with a small particle of iron oxide (SPIO) and a quantum dot (QD), and tracked these cells in the lymphatic system in mice using in vivo MRI and optical imaging. A generation-6 gadolinium-dendrimer-based MRI contrast agent (Gd-G6) was employed for visualizing regional lymphatic channels and nodes. Since Gd-G6 shortened T1 leading to high signal, whereas SPIO-labeled cancer cells greatly lowered signal, a small number of cells were simultaneously visualized within the draining lymphatic basins. One million dual-labeled cancer cells were subcutaneously injected into the paws of mice 24h prior to imaging. Then whole body images were acquired pre- and post-intracutaneous injection of Gd-G6 with 3D-T1w-FFE and balanced-FFE sequences for cancer cell tracking and MR lymphangiography. In vivo MRI clearly visualized labeled cancer cells migrating from the paw to the axillary lymph nodes using draining lymphatics. In vivo optical imaging using a fluorescence surgical microscope demonstrated tiny cancer cell clusters in the axillary lymph node with high spatial resolution. Thus, using a combination of MRI and optical imaging, it is possible to depict macro- and early micrometastases within the lymphatic system. This platform offers a versatile research tool for investigating and treating lymphatic metastases in animal models.
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DOI：
10.2217/nnm.10.49
发表时间：
2010-07
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Kosaka N;McCann TE;Mitsunaga M;Choyke PL;Kobayashi H
通讯作者：
Kobayashi H
影响因子：
11.2
作者：
Hoshida, Tohru;Isaka, Naohide;Jain, Rakesh K.
通讯作者：
Jain, Rakesh K.
影响因子：
3.3
作者：
Dahnke, H;Schaeffter, T
通讯作者：
Schaeffter, T
影响因子：
3.3
作者：
Liu, Wei;Frank, Joseph A.
通讯作者：
Frank, Joseph A.
影响因子：
46.9
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
通讯作者：
Simon, SM