18F-FDGおよび14C-FDGとのDual Tracer法を用いたラット脳糖代謝変化率の測定
18F-FDGおよび14C-FDGとのDual Tracer法を用いたラット脳糖代謝変化率の測定
复制标题
使用 18F-FDG 和 14C-FDG 双示踪法测定大鼠脑葡萄糖代谢变化率
DOI：
10.3769/radioisotopes.54.587
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
修 井上
中科院分区：
文献类型：
--
作者：
美里 網谷;桃崎 壮太郎;田口 ひとみ;理恵 細井;順 畑澤;修 井上
采用18F-FDG和14C-FDG双示踪放射自显影技术估计大鼠脑代谢捕获率的变化。示踪剂实验前3小时，大鼠右侧纹状体内灌注1μg/ l的海碱酸。大鼠静脉注射18F-FDG， 18F-FDG注射后44分钟注射14C-FDG。注射14C-FDG后1分钟处死大鼠，制作冷冻脑切片。切片在成像板上曝光1小时，获得18F-FDG图像（45分钟）。18F衰变后，同一片切片与成像板接触1周，获得14C-FDG图像（1分钟）。采用解剖法测定18F-FDG注射后1分钟和45分钟大脑皮层的放射性浓度，并将其值归一化18F-FDG和14C-FDG图像。从18F-FDG图像中减去归一化后的14C-FDG图像得到减法图像。结果表明，胃内灌注kainic酸可显著增强FDG的代谢捕获过程。用18F-FDG和14C-FDG进行双示踪放射自显影似乎有助于评估脑损伤中FDG的代谢捕获率。
The dual tracer autoradiography using 18F-FDG and 14C-FDG was applied to the estimation of changes in the metabolic trapping rate in rat brain. Rats were infused with kainic acid (1μg/μL) into the right striatum 3 hours prior to the tracer experiment. 18F-FDG was intravenously injected into the rats, and 14C-FDG was injected 44 minutes after 18F-FDG injection. The rats were decapitated at 1 minute post-injection of 14C-FDG, and frozen brain sections were prepared. The slices were exposed on imaging plate for 1 hour, and 18F-FDG images (45 minutes) were obtained. After the decay of 18F, the same slices were contacted with imaging plate for 1 week, and 14C-FDG images (1 minute) were obtained. Radioactivity concentrations in cerebral cortex at 1 minute and 45 minutes after 18F-FDG injection were determined by the dissection method, the values were used to normalize 18F-FDG and 14C-FDG image. The subtraction image was made by subtracting the normalized 14C-FDG image from the 18F-FDG image. In the results, intrastriatal infusion of kainic acid significantly enhanced the metabolic trapping process of FDG. The dual tracer autoradiography with 18F-FDG and 14C-FDG seems to be useful to assess the metabolic trapping rate of FDG in brain injury.
影响因子：
17.7
作者：
Bartlett, EJ;Brodie, JD;Schiffer, W
通讯作者：
Schiffer, W
使用 [14C]-2-脱氧葡萄糖放射自显影术评估 D1 多巴胺激动剂的体内作用。
DOI：
--
发表时间：
1989
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Trugman,JM;Arnold,WS;Touchet,N;Wooten,GF
通讯作者：
Wooten,GF
脑震荡后猫的葡萄糖利用率的区域性。
影响因子：
4.2
作者：
Hayes,RL;Katayama,Y;Jenkins,LW;Lyeth,BG;Clifton,GL;Gunter,J;Povlishock,JT;Young,HF
通讯作者：
Young,HF