Commentary on: Is preheat necessary for the measurement of 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine samples.
Commentary on: Is preheat necessary for the measurement of 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine samples.
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DOI：
10.1002/jcla.24874
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发表时间：
2023-03
影响因子：
2.7
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中科院分区：
文献类型：
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我们饶有兴趣地阅读了邹等人发表的论文1。在这篇论文中，他们使用LC-MS/MS方法研究了是否需要“预热”来精确测量氧化应激的两种尿液生物标志物8-氧- 7,8 -二氢鸟苷（8-oxoG）和8-氧- 7,8 -二氢-2 ' -脱氧鸟苷（8-oxodG）， 2。在摘要中，他们得出结论，并不总是需要预热冷冻尿液样本以从经常存在的沉淀物中释放8-oxoG和8-oxodG。然而，我们担心这种说法可能会误导读者，因为加热先前冷冻的尿液样本是确保8-oxoG和8-oxodG分析准确性和一致性的最佳策略。事实上，因为这些沉淀物可能含有感兴趣的化合物，如果沉淀物没有适当地再溶解，这可能会产生错误的数据。因此，我们希望澄清一些观点，并就分析尿液中DNA加合物的良好做法提出一些建议。重新溶解尿液中的沉淀物对于释放任何相关的加合物至关重要。因此，在完全解冻后（例如，加热到37℃）对样品进行剧烈摇动，以在分析之前重新溶解这些沉淀物是必要的。3-5作者报道，在室温（RT）下解冻30或120分钟，或在37℃下解冻15或90分钟，不影响8-oxoG和8-oxodG的测定。这是因为尿液中的沉淀物被作者完全溶解了
We read with great interest the published paper by Zou et al. 1 In this paper, they investigated whether “preheating” is necessary for the accurate measurement of two urinary biomarkers of oxidative stress 8-oxo-7, 8-dihydroguanosine (8-oxoG) and 8-oxo-7, 8-dihydro-2′-deoxyg uanosine (8-oxodG), 2 using an LC-MS/MS method. In the abstract, they concluded that it is not always necessary to preheat frozen urine samples to release 8-oxoG and 8-oxodG from precipitates that are often present. However, we are concerned that this statement may mislead readers because warming previously frozen urine samples is the best strategy to ensure the accuracy and consistency of 8-oxoG and 8-oxodG analysis. Indeed, because these precipitates may contain compounds of interest, if the precipitates are not properly redissolved, this may generate erroneous data. Therefore, we wish to clarify a number of points and provide some suggestions as to good practices when analyzing DNA adducts in urine.1. Redissolving any precipitates in urine is critical to releasing any associated adducts. Therefore, the vigorous shaking of the samples after a complete thaw (eg, warming to 37 C) to re-dissolve these precipitates before analyses is essential. 3–5 The authors reported that thawing the urine samples at room temperature (RT) for 30 or 120 min or at 37 C for 15 or 90 min did not affect the determination of 8-oxoG and 8-oxodG. This is because the precipitates in urine had been completely redissolved by the authors
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