PCR-RFLP analysis of cpDNA in the genus Abies
PCR-RFLP analysis of cpDNA in the genus Abies
复制标题
冷杉属cpDNA的PCR-RFLP分析
DOI：
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发表时间：
1999
影响因子：
5.4
通讯作者：
A. Szmidt
中科院分区：
文献类型：
--
作者：
L. Parducci;A. Szmidt
摘要本研究采用PCR-RFLP技术对松科冷杉属植物的叶绿体DNA进行分析，以确定该方法是否可用于检测冷杉属植物的种间变异，并研究变异在基因组不同区域的分布。 用特异引物扩增了冷杉属10种不同分类群的叶绿体DNA编码区和非编码区。扩增产物用几种限制性内切酶消化。结果表明，叶绿体基因组是高度可变的，在大多数的调查类群，并包含多个可变区，似乎分布在整个基因组。种诊断标记被发现的10个调查物种中的4个。出乎意料的是，在四个物种中也检测到种内变异。很可能进一步的研究，包括更大的样本量和/或更强大的方法来检测叶绿体DNA变异，将揭示该属的其他变异。
Abstract We used PCR-RFLP analysis of the chloroplast DNA of the genus Abies (family Pinaceae), to determine if the method could be employed to detect inter-specific variation in this genus and to study how the variation was distributed in different regions of the genome. Ten different chloroplast DNA regions, consisting of coding and non-coding DNA sequences, were amplified with specific primers in ten different Abies taxa. The amplification products were digested with several restriction enzymes. The results showed that the chloroplast genome is highly variable in most of the investigated taxa and contains multiple variable regions that appear to be distributed throughout the whole genome. Species-diagnostic markers were found for four of the ten investigated species. Unexpectedly, intra-specific variation was also detected in four species. It is likely that further studies, including larger sample sizes and/or more powerful methods for the detection of chloroplast DNA variation, will reveal additional variation for this genus.
DOI：
10.1073/pnas.84.24.9054
发表时间：
1987-12-01
影响因子：
11.1
作者：
WOLFE, KH;LI, WH;SHARP, PM
通讯作者：
SHARP, PM