The role of nodes in arsenic storage and distribution in rice.
The role of nodes in arsenic storage and distribution in rice.
复制标题
节点在水稻砷储存和分配中的作用
DOI：
10.1093/jxb/erv164
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发表时间：
2015-07
影响因子：
6.9
通讯作者：
Zhao FJ
中科院分区：
文献类型：
--
作者：
Chen Y;Moore KL;Miller AJ;McGrath SP;Ma JF;Zhao FJ
水稻节点是限制亚砷酸盐分布到谷物中的重要过滤器。了解水稻 (Oryza sativa L.) 中砷 (As) 的积累对于最大限度地减少砷向食物链的转移非常重要。本研究的目的是研究水稻节点在砷储存和分配中的作用。使用同步加速器 μX 射线荧光 (μ-XRF) 绘制了硅/亚砷酸盐流出载体缺陷的 lsi2 突变体及其在土壤中生长的野生型 (WT) 顶部节和节间的 As 分布图。通过定量RT-PCR研究了籽粒灌浆过程中不同组织中Lsi2的表达。将亚砷酸盐或二甲基胂酸 (DMA) 提供给切除的圆锥花序，以研究 Lsi2 和植物螯合素 (PC) 在砷分布中的作用。 μ-XRF图谱揭示了顶部节和节间不同维管束的韧皮部中砷的储存。与WT 相比，lsi2 土壤生长的植物韧皮部砷积累显着减少。 Lsi2 不仅在根中强烈表达，而且在节中也强烈表达。当切下的圆锥花序暴露于As(III)时，与WT相比，lsi2突变体在节和旗叶中分配了更多的As，而在籽粒中分配了更少的As，而DMA分布没有显着差异。 L-丁硫氨酸-亚磺酰亚胺对 PC 合成的抑制减少了顶节中 As(III) 的沉积，但增加了籽粒和旗叶中 As 的积累。结果表明，水稻节点充当过滤器，限制 As(III) 分布到谷物中。此外，Lsi2 在水稻节中 As(III) 的分布中发挥作用，植物螯合素是节中 As(III) 储存的重要化合物。
Rice nodes serve as an important filter restricting arsenite distribution to the grain. Knowledge of arsenic (As) accumulation in rice (Oryza sativa L.) is important for minimizing As transfer to the food chain. The aim of this study was to investigate the role of rice nodes in As storage and distribution. Synchrotron μX-ray fluorescence (μ-XRF) was used to map As distribution in the top node and internode of a lsi2 mutant defective in silicon/arsenite efflux carrier and its wild-type (WT) grown in soil. Lsi2 expression in different tissues during grain filling was investigated by quantitative RT-PCR. Arsenite or dimethylarsinic acid (DMA) was supplied to excised panicles to investigate the roles of Lsi2 and phytochelatins (PC) in As distribution. μ-XRF mapping revealed As storage in the phloem of different vascular bundles in the top node and internode. Soil-grown plants of lsi2 had markedly decreased As accumulation in the phloem compared with the WT. Lsi2 was strongly expressed, not only in the roots but also in the nodes. When excised panicles were exposed to As(III), the lsi2 mutant distributed more As to the node and flag leaf but less As to the grain compared with the WT, while there was no significant difference in DMA distribution. Inhibition of PC synthesis by l-buthionine-sulphoximine decreased As(III) deposition in the top node but increased As accumulation in the grain and flag leaf. The results suggest that rice nodes serve as a filter restricting As(III) distribution to the grain. Furthermore, Lsi2 plays a role in As(III) distribution in rice nodes and phytochelatins are important compounds for As(III) storage in the nodes.
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影响因子：
11.4
作者：
Li, R. Y.;Stroud, J. L.;Zhao, F. J.
通讯作者：
Zhao, F. J.
影响因子：
11.8
作者：
Li, Gang;Sun, Guo-Xin;Zhu, Yong-Guan
通讯作者：
Zhu, Yong-Guan
影响因子：
11.4
作者：
Khan, M. Asaduzzaman;Stroud, Jacqueline L.;Zhao, Fang-Jie
通讯作者：
Zhao, Fang-Jie
影响因子：
11.4
作者：
Williams, Paul N.;Villada, Antia;Meharg, Andrew A.
通讯作者：
Meharg, Andrew A.
影响因子：
4.9
作者：
KUPPELWIESER, H;FELLER, U
通讯作者：
FELLER, U