Sorting of Ion Pumps in Polarized Epithelial Cells. a
Sorting of Ion Pumps in Polarized Epithelial Cells. a
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极化上皮细胞中离子泵的分类。
DOI：
--
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发表时间：
1997
影响因子：
5.2
通讯作者：
Michael J. Caplan
中科院分区：
文献类型：
--
作者：
L. Dunbar;D. Roush;N. Courtois;T. Muth;C. Gottardi;V. Rajendran;J. Geibel;M. Kashgarian;Michael J. Caplan
P型ATP酶的生理功能不仅由其催化和调节特性决定，而且还由其在细胞的各种膜隔室中的分布决定。与极化上皮细胞介导矢量离子通量，限制P型离子泵的质膜的一个或其他不同的域在很大程度上决定了母体组织的溶质和流体的运输能力。这些各向异性分布的生物学意义通过Na，K-ATP酶驱动大多数活性上皮分泌和吸收过程的机制得到了很好的说明。在大多数上皮细胞中，Na，K-ATP酶仅限于基底外侧质膜结构域。2该膜表面位于上皮基底膜上，与细胞外液室接触，并参与与邻近上皮细胞的广泛接触。基底外侧膜通过紧密连接与顶端质膜分离，顶端质膜通常面对与体外空间拓扑连续的隔室。由钠泵产生的非平衡离子分布被次级主动转运系统利用，以驱动向上的分泌和吸收通量。通过表达不同类型的转运系统并将其分布限制在一个或另一个表面区室，上皮细胞可以使用Na，K-ATP酶的基底外侧群体来催化显著多样化的单向转运过程。为了实现P型ATP酶蛋白的极化分布，上皮细胞必须能够将新合成的离子泵靶向正确的膜表面，并在其递送后将其保留在那里。为了参与这些分类功能，P型ATP酶亚基多肽必须在其结构内编码指定其最终功能驻留位点的信息。此外，上皮细胞内的机械必须能够识别这些信息并对其信息采取行动3努力了解这些分选信号的性质和解释它们的细胞组分在很大程度上依赖于与P型ATP酶家族成员相关的广泛同源性。这种高度的结构性
The physiologic functions of a P-type ATPase are determined not only by its catalytic and regulatory properties but also by its distribution among a cell's various membranous compartments. With polarized epithelial cells that mediate vectorial ion fluxes, the restriction of P-type ion pumps to one or the other distinct domains of the plasmalemma in large measure determines the parent tissue's solute and fluid transport capacities. The biologic significance of these anisotropic distributions is well illustrated by the mechanisms through which the Na,K-ATPase drives the majority of active epithelial secretory and absorptive processes.] In most epithelial cells, the Na,K-ATPase is restricted to the basolateral plasmalemmal domain.2 This membrane surface rests on the epithelial basement membrane, is in contact with the extracellular fluid compartment, and is involved in extensive contacts with neighboring epithelial cells. The basolateral membrane is separated by tight junctions from the apical plasmalemma, which generally confronts a compartment that is topologically continuous with the extracorporeal space. The nonequilibrium ion distributions generated by the sodium pump are exploited by secondary active transport systems to drive uphill secretory and absorptive fluxes. By expressing different classes of transport systems and restricting their distributions to one or the other surface compartment, epithelial cells can use the basolateral population of the Na,K-ATPase to catalyze a remarkably diverse array of unidirectional transport processes. To achieve polarized distribution of P-type ATPase proteins, epithelial cells must be able to target newly synthesized ion pumps to the correct membrane surfaces and to retain them there following their delivery. To participate in these sorting functions, P-type ATPase subunit polypeptides must encode information within their structures that specify their sites of ultimate functional residence. Furthermore, machinery within the epithelial cell must be able to recognize this information and act on its messages3 Efforts to understand the nature of these sorting signals and of the cellular components that interpret them have largely relied on the extensive homology that relates the members of the P-type ATPase family. This high degree of structural
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静息和刺激胃壁细胞的 (H K )-ATP 酶膜的重新分布和表征。
DOI：
10.1042/bj2310641
发表时间：
1985
期刊：
The Biochemical journal
影响因子：
--
作者：
Hirst,BH;Forte,JG
通讯作者：
Forte,JG
水泡性口炎病毒糖蛋白G胞质结构域中的基底外侧靶向信号类似于多种通过一级序列相关的细胞内靶向基序，但具有不同的靶向活性。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Thomas,DC;Roth,MG
通讯作者：
Roth,MG
胃H,K-ATP酶的机制和结构。
影响因子：
18.2
作者：
Rabon,EC;Reuben,MA
通讯作者：
Reuben,MA
猪和人胃质子泵的免疫测定。
影响因子：
29.4
作者：
Smolka,A;Weinstein,WM
通讯作者：
Weinstein,WM
影响因子：
56.9
作者：
HAMMERTON, RW;KRZEMINSKI, KA;NELSON, WJ
通讯作者：
NELSON, WJ