ESR spectral transition by arteriovenous cycle in nitric oxide hemoglobin of cytokine-treated rats.
ESR spectral transition by arteriovenous cycle in nitric oxide hemoglobin of cytokine-treated rats.
复制标题
细胞因子治疗大鼠一氧化氮血红蛋白中动静脉循环的 ESR 光谱转变。
作者：
H. Kosaka;Y. Sawai;H. Sakaguchi;E. Kumura;N. Harada;M. Watanabe;T. Shiga
利用NO血红蛋白（HbNO）的电子自旋共振（ESR）信号检测大肠杆菌脂多糖（LPS）诱导大鼠产生一氧化氮（NO）。然而，它们之间的ESR光谱形状不一致。因此，我们进行了系统的研究，以澄清体内的光谱变化。首先，在肿瘤坏死因子、白细胞介素-1和/或LPS处理的大鼠中，α - no血红素在静脉血中具有明显的三线超细结构，而在动脉血中没有，在g = 6（高自旋高铁血红蛋白）区域未检测到高铁血红蛋白。第二，当接受治疗的大鼠死亡时，即使在动脉血中，三线超细结构也非常明显。第三，即使给大鼠注射亚硝酸盐形成HbNO，静脉血中HbNO的三线超细结构也比动脉血中更为明显，与高铁血红蛋白信号的出现无关。第四，全血离体研究表明，当血红蛋白氧饱和度降低时，三线超细结构呈线性增强，而当血红蛋白再氧化时，三线超细结构消失。这些结果直接证明了血红蛋白四聚体在体内从动脉周期的高亲和力状态向静脉周期的低亲和力状态的四级结构转变。这种转变使得HbNO在体内具有多种ESR谱。
Nitric oxide (NO) generation was induced in rats by Escherichia coli lipopolysaccharide (LPS) as detected by electron spin resonance (ESR) signals of NO hemoglobin (HbNO). However, there were inconsistencies in ESR spectral shape among them. We have therefore carried out a systematic study to clarify the in vivo spectral changes. First, the spectra of the alpha-NO heme species had the distinct three-line hyperfine structure in venous blood but not in arterial blood in all rats treated with tumor necrosis factor, interleukin-1, and/or LPS, and methemoglobin was not detected at the g = 6 (high-spin methemoglobin) region. Second, when the treated rats died, the three-line hyperfine structure was very distinct even in arterial blood. Third, even if HbNO was formed by injection of nitrite to rats, the three-line hyperfine structure of HbNO in venous blood was more marked than that in arterial blood, independent of the appearance of the methemoglobin signal. Fourth, an ex vivo study using whole blood demonstrated that the three-line hyperfine structure intensified lineally when O2 saturation of hemoglobin decreased but disappeared on reoxygenation of hemoglobin. These results directly demonstrate in vivo quaternary structural transition of the hemoglobin tetramer from the high-affinity state in the arterial cycle to the low-affinity state in the venous cycle. The transition makes the diverse ESR spectra of HbNO in vivo.
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影响因子：
4.7
作者：
Kosaka,H;Wishnok,JS;Miwa,M;Leaf,CD;Tannenbaum,SR
通讯作者：
Tannenbaum,SR
影响因子：
4.7
作者：
C. Leaf;J. Wishnok;S. Tannenbaum
通讯作者：
C. Leaf;J. Wishnok;S. Tannenbaum
DOI：
10.1073/pnas.84.24.9265
发表时间：
1987-12-01
影响因子：
11.1
作者：
IGNARRO, LJ;BUGA, GM;CHAUDHURI, G
通讯作者：
CHAUDHURI, G
哺乳动物硝酸盐生物合成：内毒素处理可增强 15NH3 与硝酸盐的结合。
DOI：
10.1073/pnas.80.14.4518
发表时间：
1983
影响因子：
11.1
作者：
Wagner,DA;Young,VR;Tannenbaum,SR
通讯作者：
Tannenbaum,SR
红细胞中亚硝酸盐转化为一氧化氮及其作为血红蛋白亚硝基化价杂合体的稳定性。
DOI：
--
发表时间：
1987
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Kruszyna,R;Kruszyna,H;Smith,RP;Thron,CD;Wilcox,DE
通讯作者：
Wilcox,DE