Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
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与原发性结肠癌细胞相比，转移性结肠癌细胞群含有更多的癌干细胞，对天然杀伤细胞介导的裂解具有更高的敏感性。
DOI：
10.3892/ol.2015.2918
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发表时间：
2015-04
期刊：
影响因子：
2.9
通讯作者：
Kang CD
中科院分区：
文献类型：
--
作者：
Kim GR;Ha GH;Bae JH;Oh SO;Kim SH;Kang CD
在本研究中，软琼脂克隆形成和克隆形成的癌细胞对自然杀伤（NK）细胞之间的原代结肠癌细胞（KM 12 C）和转移性结肠癌细胞（KM 12 L4 a和KM 12 SM）的敏感性进行了比较，以确定转移性癌细胞是否由更多的癌症干细胞样细胞组成，并抵抗NK细胞介导的裂解。大多数结肠癌细胞对推定的癌症干细胞标志物呈阳性，包括CD 44、CD 133和EpCAM，但KM 12 C细胞除外，其中仅约55%对CD 133呈阳性。此外，维持自我更新所必需的性别决定区Y-box 2、Nanog和八聚体结合转录因子4的表达水平在KM 12 L4 a和KM 12 SM中高于KM 12 C细胞。与此同时，在软琼脂中观察到与KM 12 C细胞相比，KM 12 L4 a和KM 12 SM的克隆形成性增加。在KM 12 L4 a和KM 12 SM细胞中，NKG 2D配体（包括主要组织相容性复合物I类多肽相关序列A/B和UL 16结合蛋白2）和死亡受体5的表达水平显著高于KM 12 C细胞。此外，结果表明与KM 12 C细胞相比，KM 12 L4 a和KM 12 SM对NK细胞介导的细胞毒性的敏感性增加。这些结果表明，转移性结肠癌细胞群可能由更多的癌症干细胞样细胞组成，并且与原发性结肠癌相比，对NK细胞介导的裂解具有更大的易感性。
In the present study, the soft agar clonogenicity and the susceptibility of clonogenic cancer cells to natural killer (NK) cells were compared between primary colon cancer cells (KM12C) and metastatic colon cancer cells (KM12L4a and KM12SM) to determine whether the metastatic cancer cells consisted of more cancer stem-like cells and were resistant to NK cell-mediated lysis. The majority of colon cancer cells were positive for putative cancer stem cell markers, including CD44, CD133 and EpCAM, with the exception of KM12C cells, of which only ~55% were positive for CD133. In addition, the expression levels of sex determining region Y-box 2, Nanog and octamer-binding transcription factor 4, which are essential for maintaining self-renewal, were higher in KM12L4a and KM12SM compared with that in KM12C cells. Consistently, an increased clonogenicity of KM12L4a and KM12SM compared with KM12C cells in soft agar was observed. The expression levels of NKG2D ligands, including major histocompatibility complex class I polypeptide-related sequence A/B and UL16 binding protein 2, and of death receptor 5 were significantly higher in KM12L4a and KM12SM than in KM12C cells. Furthermore, the results indicated an increased susceptibility of KM12L4a and KM12SM to NK cell-mediated cytotoxicity in comparison with KM12C cells. These results indicated that metastatic colon cancer cell populations may consist of more cancer stem-like cells, and have greater susceptibility to NK cell-mediated lysis compared with that of primary colon cancers.
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DOI：
10.1158/1078-0432.ccr-09-0991
发表时间：
2009-11-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
McGilvray RW;Eagle RA;Watson NF;Al-Attar A;Ball G;Jafferji I;Trowsdale J;Durrant LG
通讯作者：
Durrant LG
DOI：
10.1186/1748-717x-9-85
发表时间：
2014-03-26
期刊：
Radiation oncology (London, England)
影响因子：
--
作者：
Hennel R;Brix N;Seidl K;Ernst A;Scheithauer H;Belka C;Lauber K
通讯作者：
Lauber K
影响因子：
64.8
作者：
O'Brien, Catherine A.;Pollett, Aaron;Dick, John E.
通讯作者：
Dick, John E.
DOI：
10.1073/pnas.0703478104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Dalerba, Piero;Dylla, Scott J.;Clarke, Michael F.
通讯作者：
Clarke, Michael F.
了解和靶向癌症干细胞：治疗意义和挑战
影响因子：
8.2
作者：
Chen, Ke;Huang, Ying-hui;Chen, Ji-long
通讯作者：
Chen, Ji-long