Multi-dimensional and spatiotemporal correlative imaging at the plasma membrane of live cells to determine the continuum nano-to-micro scale lipid adaptation and collective motion
Multi-dimensional and spatiotemporal correlative imaging at the plasma membrane of live cells to determine the continuum nano-to-micro scale lipid adaptation and collective motion
复制标题
活细胞质膜的多维和时空相关成像，以确定连续纳米到微米尺度的脂质适应和集体运动
DOI：
10.1101/2021.03.02.433589
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Bernabé-Rubio M
中科院分区：
文献类型：
--
作者：
Bernabé-Rubio M
初级纤毛是一种特殊的质膜突起，具有信号传导途径的重要受体。在极化的上皮细胞中，初级纤毛在中间体残体（MBR）在顶面遇到中心体后组装。MBR周围的膜，即残留物相关膜斑（RAMP），一旦位于中心体附近，就会释放一些脂质成分，形成一个中心体相关膜斑（CAMP），睫状体膜从该中心体相关膜斑开始。RAMP在CAMP的形成过程中经历时空膜细化，CAMP在具有低横向流动性的凝聚膜中变得高度富集。为了更好地理解这一过程，我们已经开发了一种相关的成像方法，产生定量信息的脂质横向包装，其流动性和集体组装在质膜在不同的空间尺度随时间的推移。我们的工作铺平了道路的时空脂质集体组装在质膜作为一个功能决定因素在细胞生物学及其与膜的物理化学状态的直接相关性的定量理解。这些发现使我们能够更深入地了解极化上皮细胞睫状膜生物发生背后的机制。
The primary cilium is a specialized plasma membrane protrusion with important receptors for signalling pathways. In polarized epithelial cells, the primary cilium assembles after the midbody remnant (MBR) encounters the centrosome at the apical surface. The membrane surrounding the MBR, namely remnant-associated membrane patch (RAMP), once situated next to the centrosome, releases some of its lipid components to form a centrosome-associated membrane patch (CAMP) from which the ciliary membrane stems. The RAMP undergoes a spatiotemporal membrane refinement during the formation of the CAMP, which becomes highly enriched in condensed membranes with low lateral mobility. To better understand this process, we have developed a correlative imaging approach that yields quantitative information about the lipid lateral packing, its mobility and collective assembly at the plasma membrane at different spatial scales over time. Our work paves the way towards a quantitative understanding of the spatiotemporal lipid collective assembly at the plasma membrane as a functional determinant in cell biology and its direct correlation with the membrane physicochemical state. These findings allowed us to gain a deeper insight into the mechanisms behind the biogenesis of the ciliary membrane of polarized epithelial cells.
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