cAMP‐independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons
cAMP‐independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons
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非洲爪蟾蝌蚪嗅觉神经元的 cAMP 独立反应及其在嗅球神经元上的投射
DOI：
10.1113/jphysiol.2002.031914
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Schild
中科院分区：
文献类型：
--
作者：
I. Manzini;W. Rössler;D. Schild
本文报道了一种新的非洲爪哇蝌蚪嗅觉上皮切片制片法，研究了嗅觉感受器神经元(ONS)对氨基酸和Forsklin的反应。用膜片钳技术测量反应。氨基酸和Forsklin都被证明是有效的刺激物。有趣的是，一些对氨基酸有反应的鸟对Forsklin没有反应。这表明，一些氨基酸激活的转导途径不是众所周知的cAMP介导的途径。用一种新的非洲爪哇蝌蚪鼻-嗅球制剂对嗅球神经元进行钙成像，进一步阐明了cAMP介导的刺激和氨基酸刺激的不同处理。氨基酸敏感神经元向嗅球神经元的投射模式与Forsklin敏感的嗅球神经元的投射模式明显不同。氨基酸激活的嗅球神经元与Forsklin激活的嗅球神经元相比位于外侧，只有一小部分对这两种刺激有反应。磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤(IBMX)和膜通透性cAMP类似物8-(4-氯苯硫基)腺苷3‘，5’-环一磷酸(PCPT-cAMP)也激活了Forskolin激活的神经元。因此，我们得出结论，许多氨基酸的感觉转导是不依赖于cAMP的，氨基酸和cAMP介导的反应在嗅球水平上是不同的处理。
We report on responses of olfactory receptor neurons (ORNs) upon application of amino acids and forskolin using a novel slice preparation of the olfactory epithelium of Xenopus laevis tadpoles. Responses were measured using the patch‐clamp technique. Both amino acids and forskolin proved to be potent stimuli. Interestingly, a number of ORNs that responded to amino acids did not respond to forskolin. This suggests that some amino acids activate transduction pathways other than the well‐known cAMP‐mediated one. The differential processing of cAMP‐mediated stimuli on the one hand and amino acid stimuli on the other was further elucidated by calcium‐imaging of olfactory bulb neurons using a novel nose‐olfactory bulb preparation of Xenopus laevis tadpoles. The projection pattern of amino acid‐sensitive ORNs to olfactory bulb neurons differed markedly from the projection pattern of forskolin‐sensitive ORNs. Olfactory bulb neurons activated by amino acids were located laterally compared to those activated by forskolin, and only a small proportion responded to both stimuli. The ensemble of neurons activated by forskolin was also activated by the phosphodiesterase inhibitor 3‐isobutyl‐1‐methylxanthine (IBMX) and the membrane‐permeant cAMP analogue 8‐(4‐chlorophenylthio)adenosine 3′,5′‐cyclic monophosphate (pCPT‐cAMP). We therefore conclude that sensory transduction of a number of amino acids is cAMP independent, and amino acid‐ and cAMP‐mediated responses are processed differentially at the level of the olfactory bulb.
影响因子：
56.9
作者：
RESTREPO, D;MIYAMOTO, T;TEETER, JH
通讯作者：
TEETER, JH
通过存储操作的 Ca(2) 释放放大气味诱导的 Ca(2) 瞬变及其在嗅觉信号转导中的作用。
影响因子：
2.5
作者：
Zufall,F;Leinders-Zufall,T;Greer,CA
通讯作者：
Greer,CA