Single-frame 3D fluorescence microscopy with ultraminiature lensless FlatScope.
Single-frame 3D fluorescence microscopy with ultraminiature lensless FlatScope.
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DOI：
10.1126/sciadv.1701548
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发表时间：
2017-12
期刊：
影响因子：
13.6
通讯作者：
Veeraraghavan A
中科院分区：
文献类型：
--
作者：
Adams JK;Boominathan V;Avants BW;Vercosa DG;Ye F;Baraniuk RG;Robinson JT;Veeraraghavan A
FlatScope是一种无透镜显微镜，薄如信用卡，小到可以放在指尖上，可以捕获3D荧光图像。现代生物学越来越依赖荧光显微镜，这推动了对更小、更轻、更便宜的显微镜的需求。然而，传统的显微镜架构面临着一个基本的权衡：随着镜头变得越来越小，它们必须收集更少的光或成像更小的视场。为了打破设备尺寸和性能之间的这种根本权衡，我们提出了一种三维（3D）荧光成像的新概念，该概念用放置在传感器上方几百微米处的优化振幅掩模和一种有效的算法来取代透镜，该算法可以将捕获的传感器数据的单帧转换为高分辨率的3D图像。其结果是FlatScope：也许是世界上最小和最轻的显微镜。FlatScope是一种无透镜显微镜，几乎不大于图像传感器（重量约为0.2 g，厚度小于1 mm），但能够产生微米分辨率，高帧率，3D荧光电影，覆盖总体积为几立方毫米。FlatScope能够从单帧捕获的传感器数据重建完整的3D图像，这使得我们能够以比激光扫描共聚焦显微镜快大约40，000倍的速度对3D体积进行成像，同时提供相当的分辨率。我们设想，这种新的平面荧光显微镜范例将导致植入式内窥镜，最大限度地减少组织损伤，成像器阵列，覆盖大面积，可弯曲，灵活的显微镜，符合复杂的地形。
FlatScope, a lensless microscope as thin as a credit card and small enough to sit on a fingertip, captures 3D fluorescence images. Modern biology increasingly relies on fluorescence microscopy, which is driving demand for smaller, lighter, and cheaper microscopes. However, traditional microscope architectures suffer from a fundamental trade-off: As lenses become smaller, they must either collect less light or image a smaller field of view. To break this fundamental trade-off between device size and performance, we present a new concept for three-dimensional (3D) fluorescence imaging that replaces lenses with an optimized amplitude mask placed a few hundred micrometers above the sensor and an efficient algorithm that can convert a single frame of captured sensor data into high-resolution 3D images. The result is FlatScope: perhaps the world’s tiniest and lightest microscope. FlatScope is a lensless microscope that is scarcely larger than an image sensor (roughly 0.2 g in weight and less than 1 mm thick) and yet able to produce micrometer-resolution, high–frame rate, 3D fluorescence movies covering a total volume of several cubic millimeters. The ability of FlatScope to reconstruct full 3D images from a single frame of captured sensor data allows us to image 3D volumes roughly 40,000 times faster than a laser scanning confocal microscope while providing comparable resolution. We envision that this new flat fluorescence microscopy paradigm will lead to implantable endoscopes that minimize tissue damage, arrays of imagers that cover large areas, and bendable, flexible microscopes that conform to complex topographies.
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