Total alkaloids of Sophora alopecuroides- and matrine-induced reactive oxygen species impair biofilm formation of Staphylococcus epidermidis and increase bacterial susceptibility to ciprofloxacin.
Total alkaloids of Sophora alopecuroides- and matrine-induced reactive oxygen species impair biofilm formation of Staphylococcus epidermidis and increase bacterial susceptibility to ciprofloxacin.
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DOI：
10.1016/j.chmed.2020.02.006
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发表时间：
2020-10
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
--
作者：
探讨苦参总碱（TASA）和苦参碱（MT）对表皮葡萄球菌（S。epidermidis）至环丙沙星。使用2倍稀释法测定最小生物膜抑制浓度（mBIC）。生物膜的结构。用共聚焦激光扫描显微镜（CLSM）观察表皮细胞。使用DCFH-DA测定法测定细胞活性氧（ROS）。使用相应的试剂盒评估与ROS调节相关的关键因素。TASA和MT更有利于破坏S.与环丙沙星（CIP）比较，差异有统计学意义（P < 0.05）。TASA和MT不易产生对产生物膜S.表皮在亚生物膜抑制浓度（sub-BIC）TASA和MT处理后，CIP的mBIC降低了2-6倍，而从第一代到第六代，在sub-BIC CIP处理后，CIP的mBIC增加了2倍。TASA和MT能促进产膜S.表皮从第一代到第六代，亚mBIC CIP处理后ROS含量降低23%-33%，而TASA + CIP和MT + CIP组合处理后ROS含量增加7%-24%。一氧化氮（NO）作为活性氧，这与先前证实的活性氧与耐药之间的关系一致。相关的调节因子超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH）可协同维持体内氧化还原平衡。TASA和MT可增强活性氧自由基，恢复S.对环丙沙星敏感。
To investigate the mechanism by which total alkaloids of Sophora alopecuroides (TASA) and matrine (MT) impair biofilm to increase the susceptibility of Staphylococcus epidermidis (S. epidermidis) to ciprofloxacin. The minimum biofilm inhibitory concentration (mBIC) was determined using a 2-fold dilution method. Structure of biofilm of S. epidermidis was examined by Confocal Laser Scanning Microscope (CLSM). The cellular reactive oxygen species (ROS) was determined using a DCFH-DA assay. The key factors related to the regulation of ROS were accessed using respective kits. TASA and MT were more beneficial to impair biofilm of S. epidermidis than ciprofloxacin (CIP) (P < 0.05). TASA and MT were not easily developed resistance to biofilm-producing S. epidermidis. The mBIC of CIP decreased by 2–6-fold following the treatment of sub-biofilm inhibitory concentration (sub-BIC) TASA and MT, whereas the mBIC of CIP increased by 2-fold following a treatment of sub-BIC CIP from the first to sixth generations. TASA and MT can improve the production of ROS in biofilm-producing S. epidermidis. The ROS content was decreased 23%−33% following the treatment of sub-mBIC CIP, whereas ROS content increased 7%−24% following treatment with TASA + CIP and MT + CIP combination from the first to sixth generations. Nitric oxide (NO) as a ROS, which was consistent with the previously confirmed relationship between ROS and drug resistance. Related regulatory factors-superoxide dismutase (SOD) and glutathione peroxidase (GSH) could synergistically maintain the redox balance in vivo. TASA and MT enhanced reactive oxygen species to restore the susceptibility of S. epidermidis to ciprofloxacin.
生物膜形成表皮葡萄球菌对苦豆子总生物碱的响应的整体转录组变化
影响因子：
2.1
作者：
Guan CP;Luo HX;Fang HE;Zhou XZ
通讯作者：
Zhou XZ