The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway.
The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway.
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Lncrna H19 Mir-766-3P S1pr3 轴通过 Akt Mtor 通路促进牛皮癣中角质形成细胞的过度增殖和皮肤炎症
DOI：
10.1155/2021/9991175
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发表时间：
2021
影响因子：
4.6
通讯作者：
Sun Q
中科院分区：
文献类型：
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作者：
He Y;Yin X;Yan J;Li X;Sun Q
The pathogenesis of long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) are well studied in psoriasis. However, little is known about how specific lncRNAs and miRNAs affect the mechanism of psoriasis development and which pathways are involved. To explore the role of the lncRNA H19/miR-766-3p/S1PR3 axis in psoriasis. miRNA and lncRNA microarrays were performed using IL-22-induced HaCaT cells and psoriatic lesions, respectively. Fluorescence in situ hybridization and quantitative reverse-transcriptase polymerase chain reaction were used to detect the expression of miR-766-3p and lncRNA H19. Luciferase reporter assays were used to identify miR-766-3p/lncRNA H19 and miR-766-3p/S1PR3 combinations. CCK-8 and ELISA were performed to evaluate the proliferation of keratinocytes and the secretion of pro-inflammatory cytokines. Western blot analysis was used to detect the expression of S1PR3 and its downstream effector proteins. MiR-766-3p was upregulated in both HaCaT cells treated with the psoriasis-related cytokine pool (IL-17A, IL-22, IL-1 alpha, oncostatin M, and TNF-alpha) and tissues. Overexpression of miR-766-3p promoted keratinocyte proliferation and IL-17A and IL-22 secretion. LncRNA H19 and S1PR3 were demonstrably combined with miR-766-3p by luciferase reporter assay. lncRNA H19 repressed proliferation and inflammation, which were reduced by the miR-766-3p. AKT/mTOR pathway effected proliferation and inflammation by the lncRNA H19/miR-766-3p/S1PR3 axis. We established that downregulation of lncRNA H19 promoted the proliferation of keratinocytes and skin inflammation by up-regulating miR-766-3p expression levels and inhibiting activation of S1PR3 through the AKT/mTOR pathway in psoriasis.
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影响因子：
2.3
作者：
Boehncke, Wolf-Henning
通讯作者：
Boehncke, Wolf-Henning
影响因子：
3.7
作者：
Kawa Y;Nagano T;Yoshizaki A;Dokuni R;Katsurada M;Terashita T;Yasuda Y;Umezawa K;Yamamoto M;Kamiryo H;Kobayashi K;Nishimura Y
通讯作者：
Nishimura Y
影响因子：
2.4
作者：
Hu, Yi;Li, Sukai;Zou, Yonggen
通讯作者：
Zou, Yonggen
IL-22 诱导的 miR-122-5p 通过靶向 Sprouty2 促进角质形成细胞增殖
影响因子：
3.6
作者：
Jiang, Meng;Ma, Weiyuan;Sun, Qing
通讯作者：
Sun, Qing
DOI：
10.1007/978-981-15-3449-2_8
发表时间：
2020-01-01
期刊：
EPIGENETICS IN ALLERGY AND AUTOIMMUNITY
影响因子：
--
作者：
Shao, Shuai;Gudjonsson, Johann E.
通讯作者：
Gudjonsson, Johann E.