The effectiveness of the controlled release of gentamicin from polyelectrolyte multilayers in the treatment of Staphylococcus aureus infection in a rabbit bone model.
The effectiveness of the controlled release of gentamicin from polyelectrolyte multilayers in the treatment of Staphylococcus aureus infection in a rabbit bone model.
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DOI：
10.1016/j.biomaterials.2010.04.011
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发表时间：
2010-08
期刊：
影响因子：
14
通讯作者：
Hammond, Paula T.
中科院分区：
文献类型：
--
作者：
Moskowitz, Joshua S.;Blaisse, Michael R.;Samuel, Raymond E.;Hsu, Hu-Ping;Harris, Mitchel B.;Martin, Scott D.;Lee, Jean C.;Spector, Myron;Hammond, Paula T.
虽然骨科植入物的感染率很低，但所需的治疗可能涉及六周的抗生素治疗和两次额外的外科手术，这是危及生命和昂贵的，因此促使了一阶段再植入手术的发展。采用逐层沉积工艺制备含有庆大霉素的聚电解质多层膜，用于直接植入物交换手术中处理现有骨感染的设备涂层。该膜在体外三天内洗脱了约70%的有效载荷，随后持续释放药物超过四周，达到550 μg/cm2以上的总平均释放量。该涂层对金黄色葡萄球菌具有杀菌作用，降解产物对MC3T3-E1小鼠成骨前细胞一般无毒。在体内金黄色葡萄球菌骨感染模型中，将膜包覆钛种植体与未包覆钛种植体进行比较。在直接交换过程后，在第4天（p < 0.004）和第7天（p < 0.03），涂有抗菌药物的装置产生的骨匀浆感染程度明显低于未涂有抗菌药物的装置。本研究表明，自组装超薄膜涂层能够有效治疗体内实验性骨感染，为开发一种多治疗膜，优化、协同治疗疼痛、感染和骨髓炎奠定了基础。
While the infection rate of orthopedic implants is low, the required treatment, which can involve six weeks of antibiotic therapy and two additional surgical operations, is life threatening and expensive, and thus motivates the development of a one-stage re-implantation procedure. Polyelectrolyte multilayers incorporating gentamicin were fabricated using the layer-by-layer deposition process for use as a device coating to deal with an existing bone infection in a direct implant exchange operation. The films eluted about 70% of their payload in vitro during the first three days and subsequently continued to release drug for more than four additional weeks, reaching a total average release of over 550 μg/cm2. The coatings were demonstrated to be bactericidal against Staphylococcus aureus, and degradation products were generally nontoxic towards MC3T3-E1 murine preosteoblasts. Film-coated titanium implants were compared to uncoated implants in an in vivo S. aureus bone infection model. After a direct exchange procedure, the antimicrobial-coated devices yielded bone homogenates with a significantly lower degree of infection than uncoated devices at both day four (p < 0.004) and day seven (p < 0.03). This study has demonstrated that a self-assembled ultrathin film coating is capable of effectively treating an experimental bone infection in vivo and lays the foundation for development of a multi-therapeutic film for optimized, synergistic treatment of pain, infection, and osteomyelitis.
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DOI：
10.3758/bf03203630
发表时间：
1996-02-01
期刊：
BEHAVIOR RESEARCH METHODS INSTRUMENTS & COMPUTERS
影响因子：
--
作者：
Erdfelder, E;Faul, F;Buchner, A
通讯作者：
Buchner, A
影响因子：
14
作者：
Li, Bingyun;Jiang, Bingbing;Boyce, Brandon M.;Lindsey, Brock A.
通讯作者：
Lindsey, Brock A.
影响因子：
14.2
作者：
Klemm, K
通讯作者：
Klemm, K
影响因子：
5.5
作者：
Lavalle, P;Gergely, C;Picart, C
通讯作者：
Picart, C
影响因子：
11.8
作者：
Fisman, DN;Reilly, DT;Goldie, SJ
通讯作者：
Goldie, SJ