Budding Yeast Has a Minimal Endomembrane System.
Budding Yeast Has a Minimal Endomembrane System.
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DOI：
10.1016/j.devcel.2017.12.014
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发表时间：
2018-01-08
影响因子：
11.8
通讯作者：
Glick BS
中科院分区：
文献类型：
--
作者：
Day KJ;Casler JC;Glick BS
内膜系统由分泌和内吞途径组成，它们通过转运往返于高尔基体网络（trans-Golgi network，TGN）进行通信。在哺乳动物细胞中，内吞途径包括早期、晚期和再循环内体。在芽殖酵母中，已经描述了不同类型的内体，但是内吞途径的组织尚不清楚。我们进行了酵母内体标记物和内吞货物的空间和时间分析。我们的研究结果表明，酵母TGN也作为一个早期和回收内体。此外，如前所述，酵母含有晚期或前液泡内体（PVE）。内吞货物本地化后不久的TGN的内化，和操作，扰乱出口从TGN可以减缓内吞货物的PVE通道。酵母显然缺乏明显的早期内体。因此，酵母有一个简单的内吞途径，可能反映了内膜系统的祖先组织。虽然哺乳动物的内吞途径有早期、晚期和再循环内体，但酿酒酵母系统尚未解决。Day等人表明酵母只有两种类型的内体：trans-Golgi网络，作为早期和再循环内体，以及长寿的prevacuolar内体，可能反映内膜系统的祖先组织。
The endomembrane system consists of the secretory and endocytic pathways, which communicate by transport to and from the trans-Golgi network (TGN). In mammalian cells, the endocytic pathway includes early, late, and recycling endosomes. In budding yeast, different types of endosomes have been described, but the organization of the endocytic pathway has been unclear. We performed a spatial and temporal analysis of yeast endosomal markers and endocytic cargoes. Our results indicate that the yeast TGN also serves as an early and recycling endosome. In addition, as previously described, yeast contains a late or prevacuolar endosome (PVE). Endocytic cargoes localize to the TGN shortly after internalization, and manipulations that perturb export from the TGN can slow the passage of endocytic cargoes to the PVE. Yeast apparently lacks a distinct early endosome. Thus, yeast has a simple endocytic pathway that may reflect the ancestral organization of the endomembrane system. Although mammalian endocytic pathway has early, late, and recycling endosomes, the Saccharomyces cerevisiae system is unresolved. Day et al. show that yeast only has two endosome types: the trans-Golgi network, functioning as both early and recycling endosome, and the long-lived prevacuolar endosome, possibly reflecting ancestral organization of the endomembrane system.
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影响因子：
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