Fission of SNX-BAR-coated endosomal retrograde transport carriers is promoted by the dynamin-related protein Vps1.
Fission of SNX-BAR-coated endosomal retrograde transport carriers is promoted by the dynamin-related protein Vps1.
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DOI：
10.1083/jcb.201309084
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发表时间：
2014-03-03
期刊：
影响因子：
--
通讯作者：
Burd CG
中科院分区：
文献类型：
--
作者：
Chi RJ;Liu J;West M;Wang J;Odorizzi G;Burd CG
内体分选和分裂机器共同作用，生产逆行运输载体。Retromer是一种内体分选设备，它协调捕获货物并将其包装到涂有分选Nexin bar结构域蛋白(SNX-bar)的运输载体中。我们报道了从酵母内小体中分裂出反转录聚体SNX-bar包被的小管的过程是由Vps1和Mvp1促进的，Vps1是一种动态相关蛋白，定位于由反聚体SNX-bar和Mvp1修饰的内体，Mvp1是一种与人SNX8同源的SNX-bar。Mvp1在体外表现出强大的膜重塑活性，并在体内促进Vps1与内小体的结合。逆行运输载体从被逆转录单体和Mvp1包被的内体萌发，在mvp1和vps1缺失细胞中货物输出不足，但具有明显的终点；mvp1缺失细胞中货物输出延迟，而vps1缺失细胞中货物输出完全失败。结果表明，Mvp1促进Vps1介导的从内体萌发的逆转录和Mvp1包被的小管的分裂，揭示了内体分选和产生逆行运输载体的分裂机制之间的功能联系。
Endosomal sorting and fission machineries act together to produce retrograde transport carriers. Retromer is an endosomal sorting device that orchestrates capture and packaging of cargo into transport carriers coated with sorting nexin BAR domain proteins (SNX-BARs). We report that fission of retromer SNX-BAR–coated tubules from yeast endosomes is promoted by Vps1, a dynamin-related protein that localizes to endosomes decorated by retromer SNX-BARs and Mvp1, a SNX-BAR that is homologous to human SNX8. Mvp1 exhibits potent membrane remodeling activity in vitro, and it promotes association of Vps1 with the endosome in vivo. Retrograde transport carriers bud from the endosome coated by retromer and Mvp1, and cargo export is deficient in mvp1- and vps1-null cells, but with distinct endpoints; cargo export is delayed in mvp1-null cells, but cargo export completely fails in vps1-null cells. The results indicate that Mvp1 promotes Vps1-mediated fission of retromer- and Mvp1-coated tubules that bud from the endosome, revealing a functional link between the endosomal sorting and fission machineries to produce retrograde transport carriers.
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影响因子：
64.5
作者：
Boucrot E;Pick A;Çamdere G;Liska N;Evergren E;McMahon HT;Kozlov MM
通讯作者：
Kozlov MM
影响因子：
64.5
作者：
Frost A;Elgort MG;Brandman O;Ives C;Collins SR;Miller-Vedam L;Weibezahn J;Hein MY;Poser I;Mann M;Hyman AA;Weissman JS
通讯作者：
Weissman JS
影响因子：
14.9
作者：
Koh JL;Ding H;Costanzo M;Baryshnikova A;Toufighi K;Bader GD;Myers CL;Andrews BJ;Boone C
通讯作者：
Boone C
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
3.3
作者：
Luo, WJ;Chang, A
通讯作者：
Chang, A