Nerve growth factor-basic fibroblast growth factor poly-lactide co-glycolid sustained-release microspheres and the small gap sleeve bridging technique to repair peripheral nerve injury.
Nerve growth factor-basic fibroblast growth factor poly-lactide co-glycolid sustained-release microspheres and the small gap sleeve bridging technique to repair peripheral nerve injury.
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神经生长因子-碱性成纤维细胞生长因子聚丙交酯共糖醇缓释微球及小间隙套管桥接技术修复周围神经损伤
DOI：
10.4103/1673-5374.344842
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发表时间：
2023-01
影响因子：
6.1
通讯作者：
Wang, Yan-Hua
中科院分区：
文献类型：
--
作者：
Li, Ming;Xu, Ting-Min;Zhang, Dian-Ying;Zhang, Xiao-Meng;Rao, Feng;Zhan, Si-Zheng;Ma, Man;Xiong, Chen;Chen, Xiao-Feng;Wang, Yan-Hua
关键词：
我们采用小间隙袖套法制备了神经生长因子聚丙交酯-乙交酯缓释微球，用于治疗大鼠坐骨神经损伤。多种生长因子在促进周围神经损伤修复中发挥协同作用，因此，本研究在微球中加入碱性成纤维细胞生长因子，进一步促进神经再生。首先，在体外仿生微环境中，我们研制并使用药物筛选仿生微流控芯片，筛选神经生长因子/碱性成纤维细胞生长因子促进雪旺细胞再生的最佳组合。我们发现22.56 ng/mL的神经生长因子与4.29 ng/mL的碱性成纤维细胞生长因子组合对原代大鼠雪旺细胞的增殖具有最佳效果。将制备成功的神经生长因子-碱性成纤维细胞生长因子-聚乳酸-羟基乙酸共聚物缓释微球应用小间隙袖桥技术治疗大鼠坐骨神经横断损伤。与单纯上皮缝合和小间隙袖套桥接相比，小间隙袖套桥接技术联合无药缓释微球在结构和功能水平上对大鼠坐骨神经转染损伤修复的作用更强。
We previously prepared nerve growth factor poly-lactide co-glycolid sustained-release microspheres to treat rat sciatic nerve injury using the small gap sleeve technique. Multiple growth factors play a synergistic role in promoting the repair of peripheral nerve injury; as a result, in this study, we added basic fibroblast growth factors to the microspheres to further promote nerve regeneration. First, in an in vitro biomimetic microenvironment, we developed and used a drug screening biomimetic microfluidic chip to screen the optimal combination of nerve growth factor/basic fibroblast growth factor to promote the regeneration of Schwann cells. We found that 22.56 ng/mL nerve growth factor combined with 4.29 ng/mL basic fibroblast growth factor exhibited optimal effects on the proliferation of primary rat Schwann cells. The successfully prepared nerve growth factor-basic fibroblast growth factor-poly-lactide-co-glycolid sustained-release microspheres were used to treat rat sciatic nerve transection injury using the small gap sleeve bridge technique. Compared with epithelium sutures and small gap sleeve bridging alone, the small gap sleeve bridging technique combined with drug-free sustained-release microspheres has a stronger effect on rat sciatic nerve transfection injury repair at the structural and functional level.
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影响因子：
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