Adaptation of phenylalanine and tyrosine catabolic pathway to hibernation in bats.
Adaptation of phenylalanine and tyrosine catabolic pathway to hibernation in bats.
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苯丙氨酸和酪氨酸分解代谢途径对蝙蝠冬眠的适应
DOI：
10.1371/journal.pone.0062039
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Pan YH;Zhang Y;Cui J;Liu Y;McAllan BM;Liao CC;Zhang S
有些哺乳动物冬眠是为了应对恶劣的环境。虽然冬眠的哺乳动物可以代谢蛋白质，但通常在冬眠期间激活的氮代谢途径并没有完全表征。与氨基酸保存的假设相反，我们发现了氨基酸代谢的证据，包括苯丙氨酸羟化酶（PAH），尿黑酸1，2-双加氧酶（HGD），富马酰乙酰乙酸酶（FAH），参与苯丙氨酸和酪氨酸催化剂的三个关键酶在冬眠期间共同上调两个远亲物种的蝙蝠，鼠耳蝠和菊头蝠ferrumequinum。此外，这些蝙蝠肝脏中的苯丙氨酸水平在冬眠期间显著下降。因为苯丙氨酸和酪氨酸都是生糖和生酮的，这些结果表明了这种分解代谢途径在能量供应中的作用。由于这两种氨基酸的代谢酶的任何缺乏都会导致有毒代谢物的积累，这些结果也表明这些酶在冬眠期间的解毒作用。一个更高的选择性限制PAH，HPD，HGD在冬眠动物比在非冬眠动物，冬眠动物有更多的保守氨基酸残基，在这些酶比非冬眠动物。这些保守的氨基酸残基大多位于对酶的结构和活性至关重要的位置。总之，这项工作的结果提供了新的见解氮代谢和蝙蝠冬眠期间有害代谢物的去除。
Some mammals hibernate in response to harsh environments. Although hibernating mammals may metabolize proteins, the nitrogen metabolic pathways commonly activated during hibernation are not fully characterized. In contrast to the hypothesis of amino acid preservation, we found evidence of amino acid metabolism as three of five key enzymes, including phenylalanine hydroxylase (PAH), homogentisate 1,2-dioxygenase (HGD), fumarylacetoacetase (FAH), involved in phenylalanine and tyrosine catabolism were co-upregulated during hibernation in two distantly related species of bats, Myotis ricketti and Rhinolophus ferrumequinum. In addition, the levels of phenylalanine in the livers of these bats were significantly decreased during hibernation. Because phenylalanine and tyrosine are both glucogenic and ketogenic, these results indicate the role of this catabolic pathway in energy supply. Since any deficiency in the catabolism of these two amino acids can cause accumulations of toxic metabolites, these results also suggest the detoxification role of these enzymes during hibernation. A higher selective constraint on PAH, HPD, and HGD in hibernators than in non-hibernators was observed, and hibernators had more conserved amino acid residues in each of these enzymes than non-hibernators. These conserved amino acid residues are mostly located in positions critical for the structure and activity of the enzymes. Taken together, results of this work provide novel insights in nitrogen metabolism and removal of harmful metabolites during bat hibernation.
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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