Quantitative analysis of sesquiterpenes and comparison of three Curcuma wenyujin herbal medicines by micro matrix solid phase dispersion coupled with MEEKC
Quantitative analysis of sesquiterpenes and comparison of three Curcuma wenyujin herbal medicines by micro matrix solid phase dispersion coupled with MEEKC
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微基质固相分散联合MEEKC对倍半萜类化合物的定量分析及三种温郁金药材的比较
DOI：
10.1002/elps.201700454
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发表时间：
2018-02
期刊：
影响因子：
2.9
通讯作者：
楚楚
中科院分区：
文献类型：
--
作者：
楚楚
建立了一种简便、高效、环保的MSPD萃取- MEEKC分离测定姜黄文玉金中5种倍半萜类化合物的方法。首次将分子筛作为固体载体用于倍半萜类化合物的提取。考察了各参数对萃取分离效率的影响。优化条件为：用200 mg TS‐1分散样品（200 mg） 150 s，用1000 μL甲醇洗脱5种目标分析物。最后，使用含有1.3% SDS、5.0% 1 -丁醇、0.5%乙酸乙酯和10%乙腈的流动缓冲液，在pH为9.0的10 mM硼酸盐缓冲液中进行分离。结果表明，所建立的方法得到了充分的验证，并成功地应用于姜黄文榆金产地中草药中莪术素、姜黄烯醇、germacone、呋喃二烯和β -榄香烯5种倍半萜类化合物的测定。此外，根据目标化合物的含量进行分层聚类分析，以区分蒸药和非蒸药。本研究为文玉金姜黄蒸制与非蒸制中药化学成分的快速鉴别提供了一种有前景的方法。
A simple, efficient and environmental friendly method was proposed for determining five sesquiterpenoids of Curcuma wenyujin by MSPD extraction coupled with MEEKC separation. Molecular sieve was applied as a solid support for extraction of sesquiterpenoids for the first time. Various parameters affecting extraction and separation efficiency were investigated. The optimized conditions involved dispersing sample (200 mg) with 200 mg of TS‐1 for 150 s and using 1000 μL of methanol to elute five target analytes. Finally, they were well separated by using a running buffer containing 1.3% SDS, 5.0% 1‐butanol, 0.5% ethyl acetate and 10% acetonitrile in 10 mM borate buffer at pH 9.0. Consequently, the developed method was fully validated and successfully applied to determine the five sesquiterpenoids including curdine, curcumenol, germacrone, furanodiene and β‐elemene in Curcuma wenyujin origin's Chinese herbal medicines. Furthermore, hierarchical cluster analysis was performed based on the contents of target compounds for distinguishing steamed and non‐steamed drugs. The present study provided a promising method for fast investigation and discrimination of chemical difference in steam & non‐steamed Chinese medicines from Curcuma wenyujin origin.
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