Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling.
Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling.
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DOI：
10.1016/j.cmet.2018.05.025
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发表时间：
2018-08-07
期刊：
影响因子：
29
通讯作者：
Granneman JG
中科院分区：
文献类型：
--
作者：
Burl RB;Ramseyer VD;Rondini EA;Pique-Regi R;Lee YH;Granneman JG
棕色/米色脂肪细胞（BA）在白色脂肪组织（WAT）中的募集涉及脂肪细胞干细胞（ASC）的增殖和分化以及与驻留免疫细胞的密切相互作用。为了以全面和无偏倚的方式去卷积基质细胞异质性，我们在对照条件下和β3-肾上腺素能受体（ADRB 3）激活期间对来自附睾WAT（eWAT）和腹股沟WAT（iWAT）的> 33，000个基质/血管细胞进行了单细胞RNA测序（scRNA-seq）。scRNA-seq鉴定了eWAT和iWAT中不同的ASC亚群，这些亚群似乎差异性地准备进入脂肪形成途径。ADRB 3激活触发了eWAT中增殖ASC的戏剧性出现，其分化为BA可以从单个时间点推断。scRNA-seq鉴定了eWAT中的各种免疫细胞类型，包括占据脂肪形成龛的增殖巨噬细胞亚群。这些结果证明了scRNA-seq解构脂肪形成小生境的能力，并表明了驻留基质细胞亚群之间的新功能相互作用。Burl等人采用小鼠脂肪组织的单细胞RNA测序（scRNA-seq）来鉴定脂肪祖细胞和免疫细胞的不同亚群。在体内棕色脂肪形成的模型中，scRNA-seq数据用于解构脂肪形成小生境，绘制分化轨迹，并提出驻留基质细胞亚群之间的新功能相互作用。
Recruitment of brown/beige adipocytes (BA) in white adipose tissue (WAT) involves proliferation and differentiation of adipocyte stem cells (ASC) in concert with close interactions with resident immune cells. To deconvolve stromal cell heterogeneity in a comprehensive and unbiased fashion, we performed single-cell RNA sequencing (scRNA-seq) of > 33,000 stromal/vascular cells from epididymal WAT (eWAT) and inguinal WAT (iWAT) under control conditions and during β3-adrenergic receptor (ADRB3) activation. scRNA-seq identified distinct ASC subpopulations in eWAT and iWAT that appeared to be differentially poised to enter the adipogenic pathway. ADRB3 activation triggered the dramatic appearance of proliferating ASC in eWAT, whose differentiation into BA could be inferred from a single time point. scRNA-seq identified various immune cell types in eWAT, including a proliferating macrophage subpopulation that occupies adipogenic niches. These results demonstrate the power of scRNA-seq to deconstruct adipogenic niches and suggest novel functional interactions among resident stromal cell subpopulations. Burl et al. employ single-cell RNA sequencing (scRNA-seq) of mouse adipose tissue to identify distinct subpopulations of adipocyte progenitors and immune cells. In a model of in vivo brown adipogenesis, scRNA-seq data are used to deconstruct adipogenic niches, map differentiation trajectories, and suggest novel functional interactions among resident stromal cell subpopulations.
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影响因子：
14.9
作者：
Babicki S;Arndt D;Marcu A;Liang Y;Grant JR;Maciejewski A;Wishart DS
通讯作者：
Wishart DS
巨噬细胞的局部扩散有助于与肥胖相关的脂肪组织炎症。
影响因子：
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作者：
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影响因子：
7.8
作者：
Scherer, P E;Lisanti, M P;Baldini, G;Sargiacomo, M;Mastick, C C;Lodish, H F
通讯作者：
Lodish, H F
影响因子：
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通讯作者：
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影响因子：
4.6
作者：
Sun, Chengyi;Berry, William L.;Olson, Lorin E.
通讯作者：
Olson, Lorin E.