The Zinc Finger Transcription Factor Fts2 Represses the Yeast-to-Filament Transition in the Dimorphic Yeast Yarrowia lipolytica.
The Zinc Finger Transcription Factor Fts2 Represses the Yeast-to-Filament Transition in the Dimorphic Yeast Yarrowia lipolytica.
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锌指转录因子 Fts2 抑制二态性酵母解脂耶氏酵母中酵母向丝的转变
作者：
酵母-丝状体转变是二型真菌对环境刺激的重要细胞反应。除了激活剂，细胞中还有阻遏物以防止丝状体形成，当不需要阻遏时，这对于保持细胞处于酵母形式是重要的。然而，迄今为止，很少有阻遏物的表达是已知的。在这里，我们确定了一种新的阻遏物的双态酵母解脂耶氏酵母，Fts 2，这是一个C2 H2型锌指转录因子的抑制。我们发现，fts 2 Δ细胞在温和的诱导条件下表现出增加的趋化性，并在非丝诱导条件下形成丝。我们还表明，Fts 2与YlSsn 6，Tup 1-Ssn 6转录辅阻遏物的组成部分，和Fts 2-莱克萨抑制lexAop-PYlACT 1-lacZ报告Tup 1-Ssn 6依赖的方式，表明Fts 2具有转录阻遏活性和抑制基因表达通过Tup 1-Ssn 6。此外，我们发现，Fts 2抑制了大量的细胞壁蛋白基因和转录因子基因，其中一些涉及的表达反应。有趣的是，大约三分之二的Fts 2抑制基因也被Tup 1-Ssn 6抑制，这表明Fts 2可能通过Tup 1-Ssn 6抑制其靶基因。最后，我们表明，Fts 2的表达下调响应于碱性pH值和负控制Fts 2的救济促进诱导的碱性pH值的抑制。重要意义的抑制剂的抑制是重要的负调节酵母丝的转变。然而，除了在白色念珠菌，很少阻遏物的双态真菌中已知的。更重要的是，他们如何抑制服从往往不清楚。在本文中，我们报告了一个新的阻遏物的表达在Y。解脂Fts 2与C.白念珠菌，但它抑制基因表达通过转录辅阻遏Tup 1-Ssn 6，类似于CaNrg 1和Rfg 1。使用转录组测序，我们确定了Fts 2调控的整套基因，并确定了Fts 2抑制的主要靶点，这为Fts 2抑制表达的机制提供了线索。我们的研究结果有重要的意义，了解负控制的酵母丝转变的二型真菌。
The yeast-to-filament transition is an important cellular response to environmental stimulations in dimorphic fungi. In addition to activators, there are repressors in the cells to prevent filament formation, which is important to keep the cells in the yeast form when filamentation is not necessary. However, very few repressors of filamentation are known so far. Here, we identify a novel repressor of filamentation in the dimorphic yeast Yarrowia lipolytica, Fts2, which is a C2H2-type zinc finger transcription factor. We show that fts2Δ cells exhibited increased filamentation under mild filament-inducing conditions and formed filaments under non-filament-inducing conditions. We also show that Fts2 interacts with YlSsn6, component of the Tup1-Ssn6 transcriptional corepressor, and Fts2-LexA represses a lexAop-PYlACT1-lacZ reporter in a Tup1-Ssn6-dependent manner, suggesting that Fts2 has transcriptional repressor activity and represses gene expression via Tup1-Ssn6. In addition, we show that Fts2 represses a large number of cell wall protein genes and transcription factor genes, some of which are implicated in the filamentation response. Interestingly, about two-thirds of Fts2-repressed genes are also repressed by Tup1-Ssn6, suggesting that Fts2 may repress the bulk of its target genes via Tup1-Ssn6. Lastly, we show that Fts2 expression is downregulated in response to alkaline pH and the relief of negative control by Fts2 facilitates the induction of filamentation by alkaline pH. IMPORTANCE The repressors of filamentation are important negative regulators of the yeast-to-filament transition. However, except in Candida albicans, very few repressors of filamentation are known in dimorphic fungi. More importantly, how they repress filamentation is often not clear. In this paper, we report a novel repressor of filamentation in Y. lipolytica. Fts2 is not closely related in amino acid sequence to CaNrg1 and Rfg1, two major repressors of filamentation in C. albicans, yet it represses gene expression via the transcriptional corepressor Tup1-Ssn6, similar to CaNrg1 and Rfg1. Using transcriptome sequencing, we determined the whole set of genes regulated by Fts2 and identified the major targets of Fts2 repression, which provide clues to the mechanism by which Fts2 represses filamentation. Our results have important implications for understanding the negative control of the yeast-to-filament transition in dimorphic fungi.
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