Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil
Rare microbial taxa rather than phoD gene abundance determine hotspots of alkaline phosphomonoesterase activity in the karst rhizosphere soil
复制标题
稀有微生物类群而非 phoD 基因丰度决定了喀斯特根际土壤中碱性磷酸单酯酶活性的热点
DOI：
10.1007/s00374-020-01522-4
复制
发表时间：
2020-11
影响因子：
6.5
通讯作者：
Razavi Bahar S.
中科院分区：
文献类型：
--
作者：
Liu Shuang;Zhang Xinyu;Dungait Jennifer A. J.;Quine Timothy A.;Razavi Bahar S.
采用原位酶谱法研究了喀斯特土壤（pH 8.27）中3种植物（玉米、紫花苜蓿和香附子）根际和非根际土壤中碱性磷酸单酯酶（ALP）活性的空间分布。根据酶谱图像，我们确定并精确采集了ALP活性热点和非热点土壤样品，然后分析了编码碱性磷酸单酯酶的phoD基因，并对微生物群落进行了评估。结果表明：（1）不同植物类型中phoD丰度不同，苜蓿最高，玉米最低;（2）根际以变形菌属（Proteobacteria）为主，非根际以放线菌属（Actinobacteria）为主;（3）ALP活性与固氮菌的相对丰度呈正相关。但与phoD基因丰度、微生物群落丰富度和多样性呈负相关。通过酶谱分析和微生物分子生物学方法的结合，确定了根际土壤酶和微生物的活性热点，并评价了潜在活性微生物的相对贡献。我们发现，在这些热点地区的特定phoD-窝藏微生物的功能不同，取决于土壤中的植物，这对磷（P）管理在磷有限的岩溶土壤的影响。结果还表明，自由生活的N-2固定细菌（固氮菌）可能会促进ALP活性，从而强调重要的联系，耦合，土壤养分循环。
The spatial distribution of alkaline phosphomonoesterase (ALP) activity in the rhizosphere and bulk soil of three plants (Zea mays, Medicago sativa, and Cyperus rotundus) grown in a karst soil (pH 8.27) was visualized using in situ soil zymography. According to the zymogram images, we identified and precisely collected soil samples from hotspots and non-hotspots of ALP activity, and then analyzed the phoD genes that encoded alkaline phosphomonoesterase and assessed the microbial community. The results showed that (1) the phoD abundance in the plant types varied and was highest in the alfalfa and lowest in the maize; (2) Proteobacteria dominated the phoD-harboring microbes in the rhizosphere and Actinobacteria dominated the phoD-harboring microbes in the bulk soil, and (3) the ALP activity was positively correlated with the relative abundance of the diazotrophic Azotobacter, but negatively correlated with the phoD gene abundance, microbial community richness, and diversity. By coupling zymography and microbial molecular approaches, we identified hotspots of enzymatic and microbial activity in rhizosphere soil and evaluated the relative contributions from potential active microorganisms. We found that the function of specific phoD-harboring microorganisms in these hotspots differed depending on the plant in the soil, which had implications for phosphorus (P) management in P-limited karst soils. The results also suggested that free-living N-2-fixing bacteria (Azotobacter) might promote ALP activity, thereby emphasizing vital linkages between, and coupling of, the soil nutrient cycles.
登录
查看更多内容
影响因子：
5.2
作者：
Bargaz A;Lyamlouli K;Chtouki M;Zeroual Y;Dhiba D
通讯作者：
Dhiba D
影响因子：
2.6
作者：
A. Peerzada
通讯作者：
A. Peerzada
影响因子：
0.8
作者：
T. P. Salgado;R. Pitelli;P. Alves;F. Salvador;A. S. Nunes
通讯作者：
T. P. Salgado;R. Pitelli;P. Alves;F. Salvador;A. S. Nunes
影响因子：
--
作者：
J. Iqbal;S. Hussain;Ashfaq Ali;A. Javaid
通讯作者：
J. Iqbal;S. Hussain;Ashfaq Ali;A. Javaid
影响因子：
4.8
作者：
S. Reed;T. Seastedt;C. Mann;K. Suding;A. Townsend;Karie L. Cherwin
通讯作者：
S. Reed;T. Seastedt;C. Mann;K. Suding;A. Townsend;Karie L. Cherwin