High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities.
High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities.
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DOI：
10.1093/aob/mcs245
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发表时间：
2013-07
期刊：
影响因子：
4.2
通讯作者：
Hammond JP
中科院分区：
文献类型：
--
作者：
Shi L;Shi T;Broadley MR;White PJ;Long Y;Meng J;Xu F;Hammond JP
土壤中的磷（Pi）缺乏是全球作物生长的主要限制因素。低磷条件下的植物生长与根构型性状相关，因此有可能选择这些性状用于作物改良。本研究的目的是在低磷（LP）和高磷（HP）条件下对甘蓝型油菜根系构型特征进行表征，并检测与这些特征相关的数量性状基因座（QTL）。在B的双单倍体（DH）作图群体（n = 190）的幼苗中表征了根构型性状。napus‘Tapidor’ב宁优7号’（TNDH）]的遗传多样性。在LP和HP条件下，测定了萌发12 d后的初生根长（PRL）、侧根长（LRL）、侧根数（LRN）、侧根密度（LRD）和生物量性状。在一般情况下，根和生物量性状在LP和HP条件下高度相关。在LP和HP可用度下，‘宁优7号’的LRL、LRN和LRD均大于‘Tapidor’，但PRL小于‘Tapidor’。LRN、LRD和LP利用率下的生物量性状的一组极显著QTL位于染色体A03; PRL的QTL位于染色体A07和C 06。芸苔属植物的高通量表型分析可用于鉴定与地上部生物量相关的根构型性状。这些性状可用于作物改良策略。在LP和HP条件下与根性状相关的QTL的鉴定为植物耐磷缺乏的遗传基础提供了进一步的见解，这些QTL值得进一步研究。
Phosphate (Pi) deficiency in soils is a major limiting factor for crop growth worldwide. Plant growth under low Pi conditions correlates with root architectural traits and it may therefore be possible to select these traits for crop improvement. The aim of this study was to characterize root architectural traits, and to test quantitative trait loci (QTL) associated with these traits, under low Pi (LP) and high Pi (HP) availability in Brassica napus. Root architectural traits were characterized in seedlings of a double haploid (DH) mapping population (n = 190) of B. napus [‘Tapidor’ × ‘Ningyou 7’ (TNDH)] using high-throughput phenotyping methods. Primary root length (PRL), lateral root length (LRL), lateral root number (LRN), lateral root density (LRD) and biomass traits were measured 12 d post-germination in agar at LP and HP. In general, root and biomass traits were highly correlated under LP and HP conditions. ‘Ningyou 7’ had greater LRL, LRN and LRD than ‘Tapidor’, at both LP and HP availability, but smaller PRL. A cluster of highly significant QTL for LRN, LRD and biomass traits at LP availability were identified on chromosome A03; QTL for PRL were identified on chromosomes A07 and C06. High-throughput phenotyping of Brassica can be used to identify root architectural traits which correlate with shoot biomass. It is feasible that these traits could be used in crop improvement strategies. The identification of QTL linked to root traits under LP and HP conditions provides further insights on the genetic basis of plant tolerance to P deficiency, and these QTL warrant further dissection.
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DOI：
10.1002/9780470168035.ch7
发表时间：
2007-01-01
期刊：
Plant Breeding Reviews
影响因子：
--
作者：
Jain, A.;Vasconcelos, M. J.;Sahi, S. V.
通讯作者：
Sahi, S. V.
影响因子：
7.3
作者：
Al-Ghazi, Y;Muller, B;Doumas, P
通讯作者：
Doumas, P
影响因子：
7.4
作者：
Chin, Joong Hyoun;Gamuyao, Rico;Heuer, Sigrid
通讯作者：
Heuer, Sigrid
影响因子：
4.3
作者：
Jose Camacho-Cristobal, Juan;Rexach, Jesus;Doumas, Patrick
通讯作者：
Doumas, Patrick
DOI：
10.1007/978-3-642-15271-9_10
发表时间：
2011-01-01
期刊：
PHOSPHORUS IN ACTION: BIOLOGICAL PROCESSES IN SOIL PHOSPHORUS CYCLING
影响因子：
--
作者：
George, Timothy S.;Fransson, Ann-Mari;White, Philip J.
通讯作者：
White, Philip J.