Emergence of chemical biology approaches to the RNAi/miRNA pathway.
Emergence of chemical biology approaches to the RNAi/miRNA pathway.
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DOI：
10.1016/j.chembiol.2010.05.014
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发表时间：
2010-06-25
影响因子：
--
通讯作者：
Jin P
中科院分区：
文献类型：
--
作者：
Li Y;He C;Jin P
RNA干扰（RNAi）是一种高度保守的机制，它利用小的非编码RNA在转录后沉默基因表达。RNAi是真核细胞基因调控的主要途径之一。虽然RNAi/miRNA途径的主要成分已经被确定，但调节RNAi/miRNA途径活性的分子机制仅在最近几年才开始出现。最近，已经开发了用于监测RNAi/miRNA途径的活性的高通量报告基因测定，并用于概念验证中试筛选。RNAi/miRNA通路的抑制剂和激活剂都已被发现，尽管仍处于起步阶段，但利用高通量化学筛选的化学生物学方法将为深入研究RNAi/miRNA通路以及开发基于RNAi或miRNA的新型治疗干预开辟新的途径。
RNA interference (RNAi) is a well-conserved mechanism that uses small noncoding RNAs to silence gene expression posttranscriptionally. Gene regulation by RNAi is now recognized as one of the major regulatory pathways in eukaryotic cells. Although the main components of the RNAi/miRNA pathway have been identified, the molecular mechanisms regulating the activity of the RNAi/miRNA pathway have only begun to emerge within the last couple of years. Recently, high-throughput reporter assays to monitor the activity of the RNAi/miRNA pathway have been developed and used for proof-of-concept pilot screens. Both inhibitors and activators of the RNAi/miRNA pathway have been found. Although still in its infancy, a chemical biology approach using high-throughput chemical screens should open up a new avenue for dissecting the RNAi/miRNA pathway, as well as developing novel RNAi- or miRNA-based therapeutic interventions.
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