Covalent-bond-based immobilization approaches for single-molecule fluorescence.
Covalent-bond-based immobilization approaches for single-molecule fluorescence.
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DOI:
10.1002/cbic.200900640
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发表时间:
2009-12-14
期刊:
影响因子:
3.2
通讯作者:
Rueda, David
中科院分区:
文献类型:
--
作者:
Aleman, Elvin A.;Pedini, Heidi S.;Rueda, David
关键词:
INH: We report two new approaches, using click-chemistry and disulfide bond bridges, to surface-immobilize nucleic acids for single-molecule fluorescence experiments using covalent bonds and self-assembled monolayers. Both approaches are specific and yield comparable results to the avidin-biotin linkage, but offer new surface chemical properties that might be advantageous to prevent non-specific binding of biopolymers to the surface and to expand the range of fluorescent probes that can be employed in single-molecule studies.
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