A genomic reappraisal of symbiotic function in the aphid/Buchnera symbiosis: reduced transporter sets and variable membrane organisations.
A genomic reappraisal of symbiotic function in the aphid/Buchnera symbiosis: reduced transporter sets and variable membrane organisations.
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DOI：
10.1371/journal.pone.0029096
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Rahbé Y
中科院分区：
文献类型：
--
作者：
Charles H;Balmand S;Lamelas A;Cottret L;Pérez-Brocal V;Burdin B;Latorre A;Febvay G;Colella S;Calevro F;Rahbé Y
蚜虫生布氏菌是一种专性共生细菌，通过补充蚜虫独有的韧皮部汁液饮食来维持蚜虫的生理。在这项研究中，我们重新评估了不同Buchnera菌株的运输功能，从蚜虫Acyrthosiphon豌豆，麦二叉蚜，百宗黄连木和Cinara雪松，使用重新注释的跨膜蛋白加上探索他们的代谢网络。虽然代谢分析显示宿主和细菌之间的相互依赖性很高，但我们在这里证明，与自由生活的细菌相比，Buchnera中的运输是由低转运蛋白多样性保证的，主要是基于一些一般的转运蛋白，其中一些可能已经失去了底物特异性。此外，在所研究的四种菌株中，观察到内膜进口商的惊人缺乏。在Buchnera，运输功能已经形成了独特的选择性限制发生在蚜虫科谱系。Buchnera从A.豌豆S.禾本科植物具有三膜系统和对应于大多数化合物类别的类似的转运体组。透射电子显微镜观察和共聚焦显微镜分析的细胞内pH值字段显示，Buchnera不显示任何典型的结构和性能中观察到的综合细胞器。来自B的Buchnera。阿月浑子似乎具有独特的双膜系统，因此，失去了所有的外膜整合蛋白。最后，C. cedri揭示了一个非常差的转运蛋白库，几乎没有ATP驱动的主动运输离开，尽管祖先的三膜系统的明显持久性。
Buchnera aphidicola is an obligate symbiotic bacterium that sustains the physiology of aphids by complementing their exclusive phloem sap diet. In this study, we reappraised the transport function of different Buchnera strains, from the aphids Acyrthosiphon pisum, Schizaphis graminum, Baizongia pistaciae and Cinara cedri, using the re-annotation of their transmembrane proteins coupled with an exploration of their metabolic networks. Although metabolic analyses revealed high interdependencies between the host and the bacteria, we demonstrate here that transport in Buchnera is assured by low transporter diversity, when compared to free-living bacteria, being mostly based on a few general transporters, some of which probably have lost their substrate specificity. Moreover, in the four strains studied, an astonishing lack of inner-membrane importers was observed. In Buchnera, the transport function has been shaped by the distinct selective constraints occurring in the Aphididae lineages. Buchnera from A. pisum and S. graminum have a three-membraned system and similar sets of transporters corresponding to most compound classes. Transmission electronic microscopic observations and confocal microscopic analysis of intracellular pH fields revealed that Buchnera does not show any of the typical structures and properties observed in integrated organelles. Buchnera from B. pistaciae seem to possess a unique double membrane system and has, accordingly, lost all of its outer-membrane integral proteins. Lastly, Buchnera from C. cedri revealed an extremely poor repertoire of transporters, with almost no ATP-driven active transport left, despite the clear persistence of the ancestral three-membraned system.
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影响因子：
14.9
作者：
Caspi R;Altman T;Dale JM;Dreher K;Fulcher CA;Gilham F;Kaipa P;Karthikeyan AS;Kothari A;Krummenacker M;Latendresse M;Mueller LA;Paley S;Popescu L;Pujar A;Shearer AG;Zhang P;Karp PD
通讯作者：
Karp PD
影响因子：
4.3
作者：
Cottret, Ludovic;Milreu, Paulo Vieira;Sagot, Marie-France
通讯作者：
Sagot, Marie-France
影响因子：
14.9
作者：
Cottret L;Wildridge D;Vinson F;Barrett MP;Charles H;Sagot MF;Jourdan F
通讯作者：
Jourdan F
影响因子：
3.8
作者：
Bouvaine, Sophie;Boonham, Neil;Douglas, Angela E.
通讯作者：
Douglas, Angela E.
影响因子：
2.2
作者：
DOUGLAS, AE;PROSSER, WA
通讯作者：
PROSSER, WA