Comprehensive Analysis of Arabinogalactan Protein-Encoding Genes Reveals the Involvement of Three BrFLA Genes in Pollen Germination in Brassica rapa.
Comprehensive Analysis of Arabinogalactan Protein-Encoding Genes Reveals the Involvement of Three BrFLA Genes in Pollen Germination in Brassica rapa.
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阿拉伯半乳聚糖蛋白编码基因的综合分析揭示了三个 BrFLA 基因参与白菜花粉萌发
DOI：
10.3390/ijms222313142
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发表时间：
2021-12-05
影响因子：
5.6
通讯作者：
Lin S
中科院分区：
文献类型：
--
作者：
Huang H;Miao Y;Zhang Y;Huang L;Cao J;Lin S
阿拉伯半乳聚糖蛋白（AGPs）是一个富含羟脯氨酸的糖蛋白超家族，广泛参与植物的生长发育。尚未对大白菜（Brassica rapa ssp.）chinensis）。在此，我们在B中鉴定了总共293个推定的AGP编码基因。包括25个经典AGP、3个富含赖氨酸的AGP、30个AG肽、36个fasciclin-like AGP（FLAs）、59个phytocyanin-like AGP、33个xylogen-like AGP、102个其他嵌合AGP、2个非经典AGP和3个AGP/extensin杂交体。对它们的蛋白质结构、系统发育关系、染色体定位和基因重复状况进行了综合分析。基于RNA测序数据，我们发现73个AGP基因在B的不育株和可育株的花芽中至少在一个发育阶段差异表达。rapa，表明AGPs在男性生殖发育中的潜在作用。我们进一步鉴定了BrFLA 2、BrFLA 28和BrFLA 32，这三个弗拉成员特别在花药、花粉粒和花粉管中表达。BrFLA 2、BrFLA 28和BrFLA 32是高湿条件下花粉萌发的关键因子。我们的研究极大地扩展了B中AGPs的功能。揭示了弗拉亚科的三个成员在花粉萌发中的作用。
Arabinogalactan proteins (AGPs) are a superfamily of hydroxyproline-rich glycoproteins that are massively glycosylated, widely implicated in plant growth and development. No comprehensive analysis of the AGP gene family has been performed in Chinese cabbage (Brassica rapa ssp. chinensis). Here, we identified a total of 293 putative AGP-encoding genes in B. rapa, including 25 classical AGPs, three lysine-rich AGPs, 30 AG-peptides, 36 fasciclin-like AGPs (FLAs), 59 phytocyanin-like AGPs, 33 xylogen-like AGPs, 102 other chimeric AGPs, two non-classical AGPs and three AGP/extensin hybrids. Their protein structures, phylogenetic relationships, chromosomal location and gene duplication status were comprehensively analyzed. Based on RNA sequencing data, we found that 73 AGP genes were differentially expressed in the floral buds of the sterile and fertile plants at least at one developmental stage in B. rapa, suggesting a potential role of AGPs in male reproductive development. We further characterized BrFLA2, BrFLA28 and BrFLA32, three FLA members especially expressed in anthers, pollen grains and pollen tubes. BrFLA2, BrFLA28 and BrFLA32 are indispensable for the proper timing of pollen germination under high relative humidity. Our study greatly extends the repertoire of AGPs in B. rapa and reveals a role for three members of the FLA subfamily in pollen germination.
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Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
通讯作者：
Bateman, Alex
影响因子：
3.7
作者：
Dong X;Feng H;Xu M;Lee J;Kim YK;Lim YP;Piao Z;Park YD;Ma H;Hur Y
通讯作者：
Hur Y
芜菁花粉发育和受精过程中长非编码RNA的系统鉴定
影响因子：
7.2
作者：
Huang, Li;Dong, Heng;Cao, Jiashu
通讯作者：
Cao, Jiashu
影响因子：
9.4
作者：
Herve, Cecile;Simeon, Amandine;Kloareg, Bernard
通讯作者：
Kloareg, Bernard
影响因子：
11.2
作者：
Bartels, Desiree;Baumann, Alexander;Classen, Birgit
通讯作者：
Classen, Birgit