Comprehensive analysis of genic male sterility-related genes in Brassica rapa using a newly developed Br300K oligomeric chip.
Comprehensive analysis of genic male sterility-related genes in Brassica rapa using a newly developed Br300K oligomeric chip.
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DOI：
10.1371/journal.pone.0072178
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hur Y
中科院分区：
文献类型：
--
作者：
Dong X;Feng H;Xu M;Lee J;Kim YK;Lim YP;Piao Z;Park YD;Ma H;Hur Y
目的鉴定大白菜（Brassica rapa ssp.） pekinensis），花芽转录组分析进行了使用 B。具有300，000个探针的rapa微阵列（Br 300 K）。在Br 300 K微阵列上的47，548个克隆中，在3' 150 bp区域内有7个长度为60 nt的探针，总共有10，622个基因在可育和不育花蕾中差异表达; 4，774个和5，848个基因在可育和不育花蕾中分别上调超过2倍。然而，1，413和199个基因的表达分别表现出可育和不育芽的特异性特征。在可育芽中特异表达的基因，可能是GMS相关基因，包括几个拟南芥雄性不育相关基因的同源物，与细胞壁及其表面蛋白合成相关的基因，花粉壁和外壳成分，信号成分和营养供应。然而，大多数花粉发育的早期基因，原基和胼胝质形成的基因，花粉成熟和花药开裂的基因表现出可育和不育芽之间的表达没有差异。一些与拟南芥花粉发育相关的已知基因显示出与本研究中所见相似的表达模式，而另一些则没有。BrbHLH 89和BrMYP 99是推定的GMS基因。此外，17个新基因仅在 B。rapa基因仅在可育芽中特异性高表达，可能与雄性育性有关。这些结果表明，白菜核不育可能是由控制绒毡层减数分裂后发育的基因所控制的，这些基因与已知的拟南芥雄性不育基因不同。
To identify genes associated with genic male sterility (GMS) that could be useful for hybrid breeding in Chinese cabbage ( Brassica rapa ssp. pekinensis ), floral bud transcriptome analysis was carried out using a B . rapa microarray with 300,000 probes (Br300K). Among 47,548 clones deposited on a Br300K microarray with seven probes of 60 nt length within the 3' 150 bp region, a total of 10,622 genes were differentially expressed between fertile and sterile floral buds; 4,774 and 5,848 genes were up-regulated over 2-fold in fertile and sterile buds, respectively. However, the expression of 1,413 and 199 genes showed fertile and sterile bud-specific features, respectively. Genes expressed specifically in fertile buds, possibly GMS-related genes, included homologs of several Arabidopsis male sterility-related genes, genes associated with the cell wall and synthesis of its surface proteins, pollen wall and coat components, signaling components, and nutrient supplies. However, most early genes for pollen development, genes for primexine and callose formation, and genes for pollen maturation and anther dehiscence showed no difference in expression between fertile and sterile buds. Some of the known genes associated with Arabidopsis pollen development showed similar expression patterns to those seen in this study, while others did not. BrbHLH89 and BrMYP99 are putative GMS genes. Additionally, 17 novel genes identified only in B . rapa were specifically and highly expressed only in fertile buds, implying the possible involvement in male fertility. All data suggest that Chinese cabbage GMS might be controlled by genes acting in post-meiotic tapetal development that are different from those known to be associated with Arabidopsis male sterility.
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