Spatiotemporal Tracking of Brain-Tumor-Associated Myeloid Cells in Vivo through Optical Coherence Tomography with Plasmonic Labeling and Speckle Modulation.
Spatiotemporal Tracking of Brain-Tumor-Associated Myeloid Cells in Vivo through Optical Coherence Tomography with Plasmonic Labeling and Speckle Modulation.
复制标题
通过具有等离子体标记和散斑调制的光学相干断层扫描对体内脑肿瘤相关骨髓细胞进行时空追踪。
DOI：
10.1021/acsnano.9b02656
复制
发表时间：
2019
期刊：
影响因子：
17.1
通讯作者：
delaZerda,Adam
中科院分区：
文献类型：
--
作者：
SoRelle,ElliottDaniel;Yecies,DerekWilliam;Liba,Orly;Bennett,FrederickChristian;Graef,ClausMoritz;Dutta,Rebecca;Mitra,Siddhartha;Joubert,Lydia-Marie;Cheshier,Samuel;Grant,GeraldA;delaZerda,Adam
就其性质而言，肿瘤对免疫系统在细胞识别和反应协调方面提出了一系列深刻的挑战。最近的研究表明，白细胞亚群，特别是肿瘤相关巨噬细胞（tam），可以对各种癌症免疫治疗策略的疗效产生实质性影响。为了更好地研究和理解tam在确定免疫治疗结果中的作用，必须克服在相关实体瘤活体动物模型中动态监测感兴趣的单细胞相关的重大技术挑战。然而，结合时空分辨率、细胞特异性对比度和足够的组织深度信噪比的成像技术是非常有限的。在这里，我们描述了一种基于散斑调制光学相干断层扫描（SM-OCT）和优化造影剂的光谱散射的高分辨率，宽视场，tam纵向成像的方法。该方法提高了OCT检测灵敏度和降低了噪声，使得基于OCT的高分辨率观察活体动物中特定宿主谱系的单个细胞成为可能。我们发现，在多形性胶质母细胞瘤小鼠体内原位模型中，具有窄带增强散射截面的大金纳米棒（LGNRs）可以选择性地标记tam并激活小胶质细胞。我们展示了这些骨髓亚群中细胞迁移的近实时跟踪。本文报道的固有时空分辨率、成像深度和对比灵敏度可能有助于在体内单细胞水平上详细研究tam和其他白细胞的基本行为，包括肿瘤内分布异质性和调节肿瘤增殖的作用。
By their nature, tumors pose a set of profound challenges to the immune system with respect to cellular recognition and response coordination. Recent research indicates that leukocyte subpopulations, especially tumor-associated macrophages (TAMs), can exert substantial influence on the efficacy of various cancer immunotherapy treatment strategies. To better study and understand the roles of TAMs in determining immunotherapeutic outcomes, significant technical challenges associated with dynamically monitoring single cells of interest in relevant live animal models of solid tumors must be overcome. However, imaging techniques with the requisite combination of spatiotemporal resolution, cell-specific contrast, and sufficient signal-to-noise at increasing depths in tissue are exceedingly limited. Here we describe a method to enable high-resolution, wide-field, longitudinal imaging of TAMs based on speckle-modulating optical coherence tomography (SM-OCT) and spectral scattering from an optimized contrast agent. The approach’s improvements to OCT detection sensitivity and noise reduction enabled high-resolution OCT-based observation of individual cells of a specific host lineage in live animals. We found that large gold nanorods (LGNRs) that exhibit a narrow-band, enhanced scattering cross-section can selectively label TAMs and activate microglia in anin vivoorthotopic murine model of glioblastoma multiforme. We demonstrated near real-time tracking of the migration of cells within these myeloid subpopulations. The intrinsic spatiotemporal resolution, imaging depth, and contrast sensitivity reported herein may facilitate detailed studies of the fundamental behaviors of TAMs and other leukocytes at the single-cell levelin vivo, including intratumoral distribution heterogeneity and roles in modulating cancer proliferation.
登录
查看更多内容
影响因子：
38.3
作者：
通讯作者：
--
利用金属纳米粒子中的等离激元共振散射进行双波段光谱光频域成像。
影响因子：
3.6
作者：
Tae Shik Kim;S. Jang;Nuri Oh;Yongjoo Kim;T. Park;Jiho Park;W. Oh
通讯作者：
W. Oh
影响因子：
4
作者：
Y. Winetraub;E. SoRelle;O. Liba;A. de la Zerda
通讯作者：
Y. Winetraub;E. SoRelle;O. Liba;A. de la Zerda
影响因子：
4.4
作者：
A. L. Ford;A. Goodsall;W. Hickey;J. Sedgwick
通讯作者：
A. L. Ford;A. Goodsall;W. Hickey;J. Sedgwick
影响因子：
1.7
作者：
Ripandelli, G;Coppe, A M;Stirpe, M
通讯作者：
Stirpe, M