Tyrosinase-based ruthenium dispersed carbon paste biosensor for phenols.
Tyrosinase-based ruthenium dispersed carbon paste biosensor for phenols.
复制标题
基于酪氨酸酶的钌分散碳糊苯酚生物传感器。
DOI：
10.1016/0956-5663(94)80009-x
复制
发表时间：
1994
影响因子：
12.6
通讯作者：
D. Lopez
中科院分区：
文献类型：
--
作者：
J. Wang;F. Lu;D. Lopez
描述了基于将酪氨酸酶掺入金属分散的碳糊基质中的酚类化合物的高灵敏度生物传感器。特别是，掺钌碳糊对于还原酶促生成的醌类物质表现出优异的电催化活性。因此，在 0·0 V（相对于 Ag/AgCl 参考）下可以方便地定量微摩尔浓度，与传统的碳糊酪氨酸酶生物传感器相比，响应大大增强。探讨了各种实验变量的影响。阐述了酚类化合物的快速、灵敏且可重复的流动注射检测。据报道，富含酪氨酸酶的植物组织电极也有类似的改进。
Highly sensitive biosensors for phenolic compounds, based on the incorporation of the enzyme tyrosinase within metal-dispersed carbon paste matrices are described. In particular, ruthernium-doped carbon pastes display excellent electrocatalytic activity for the reduction of the enzymatically generated quinone species. Hence, convenient quantitation of micromolar concentrations is feasible at 0·0 V (vs. Ag/AgCl reference), with a greatly enhanced response compared to conventional carbon-paste tyrosinase biosensors. The influence of various experimental variables is explored. Fast, sensitive and reproducible flow injection detection of phenolic compounds is illustrated. Analogous improvements are reported for tyrosinase-rich plant tissue electrodes.
用于制备酶电极的硅脂固定化方法。
DOI：
10.1039/an9891401427
发表时间：
1989
期刊：
The Analyst
影响因子：
--
作者：
Connor,MP;Sanchez,J;Wang,J;Smyth,MR;Mannino,S
通讯作者：
Mannino,S