Depletion plays a pivotal role in self-incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes.
Depletion plays a pivotal role in self-incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes.
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DOI：
10.1111/nph.18350
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发表时间：
2022-12
期刊：
影响因子：
9.4
通讯作者：
Bosch, Maurice
中科院分区：
文献类型：
--
作者：
Wang, Ludi;Lin, Zongcheng;Carli, Jose;Gladala-Kostarz, Agnieszka;Davies, Julia M.;Franklin-Tong, Vernonica E.;Bosch, Maurice
关键词：
自交不亲和性（SI）涉及授粉过程中的特定相互作用，以拒绝不亲和（“自我”）花粉，防止被子植物的近亲繁殖。在经历虞美人SI反应的花粉中观察到的关键事件是点状F-肌动蛋白灶的形成。花粉管生长严重依赖于能量，但在SI期间尚未直接测量花粉管中的ATP水平。在这里，我们使用表达罂粟花粉S-决定簇的转基因拟南芥系来研究ATP水平、细胞溶质pH（[pH]cyt）和肌动蛋白细胞骨架改变之间的可能联系。我们首次发现，SI触发快速和显着的ATP耗竭花粉管。人工消耗ATP引发胞质酸化和肌动蛋白聚集体的形成。我们还确定在体内，证据的阈值[pH]细胞因子为5.8肌动蛋白病灶的形成。成像显示，SI刺激邻近质膜的酸性胞质斑块。总之，本研究提供的证据表明，ATP耗竭起着关键作用，SI上游的程序性细胞死亡，并揭示了细胞能量状态，胞质酸化和改变的肌动蛋白细胞骨架在调节罂粟SI在花粉管之间的联系。另请参阅张对本文的评论，236：1625-1628。
Self‐incompatibility (SI) involves specific interactions during pollination to reject incompatible (‘self’) pollen, preventing inbreeding in angiosperms. A key event observed in pollen undergoing the Papaver rhoeas SI response is the formation of punctate F‐actin foci. Pollen tube growth is heavily energy‐dependent, yet ATP levels in pollen tubes have not been directly measured during SI. Here we used transgenic Arabidopsis lines expressing the Papaver pollen S‐determinant to investigate a possible link between ATP levels, cytosolic pH ([pH]cyt) and alterations to the actin cytoskeleton. We identify for the first time that SI triggers a rapid and significant ATP depletion in pollen tubes. Artificial depletion of ATP triggered cytosolic acidification and formation of actin aggregates. We also identify in vivo, evidence for a threshold [pH]cyt of 5.8 for actin foci formation. Imaging revealed that SI stimulates acidic cytosolic patches adjacent to the plasma membrane. In conclusion, this study provides evidence that ATP depletion plays a pivotal role in SI upstream of programmed cell death and reveals a link between the cellular energy status, cytosolic acidification and alterations to the actin cytoskeleton in regulating Papaver SI in pollen tubes. See also the Commentary on this article by Cheung, 236: 1625–1628.
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DOI：
10.1073/pnas.1613499114
发表时间：
2017-06-13
影响因子：
11.1
作者：
Barbez, Elke;Duenser, Kai;Busch, Wolfgang
通讯作者：
Busch, Wolfgang
DOI：
10.1083/jcb.144.3.483
发表时间：
1999-02-08
期刊：
The Journal of cell biology
影响因子：
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作者：
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通讯作者：
Hepler PK
影响因子：
7.4
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Palmgren, Michael
影响因子：
64.8
作者：
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通讯作者：
Franklin-Tong, Vernonica E.
DOI：
10.1016/j.bbrc.2008.04.135
发表时间：
2008-07-11
影响因子：
3.1
作者：
Arii, Yusuke;Hatori, Kuniyuki
通讯作者：
Hatori, Kuniyuki