Linking stomatal traits and expression of slow anion channel genes HvSLAH1 and HvSLAC1 with grain yield for increasing salinity tolerance in barley.
Linking stomatal traits and expression of slow anion channel genes HvSLAH1 and HvSLAC1 with grain yield for increasing salinity tolerance in barley.
复制标题
将气孔性状以及慢阴离子通道基因 HvSLAH1 和 HvSLAC1 的表达与谷物产量联系起来，以提高大麦的耐盐性
DOI：
10.3389/fpls.2014.00634
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发表时间：
2014
影响因子：
5.6
通讯作者：
Chen ZH
中科院分区：
文献类型：
--
作者：
Liu X;Mak M;Babla M;Wang F;Chen G;Veljanoski F;Wang G;Shabala S;Zhou M;Chen ZH
土壤盐渍化是世界上许多地区的环境和农业问题。大麦耐盐育种的关键之一在于更好地理解气孔调节的遗传控制。我们采用了一系列的生理（气孔测定，气体交换，系统发育分析，QTL分析），和分子技术（RT-PCR和qPCR），调查气孔行为和基因型变异大麦品种和遗传群体在四个实验试验。建立了一套较为有效、可靠的方法，用于大量品种和遗传系气孔行为的表征。此外，我们还发现大麦在盐胁迫下的气体交换和气孔性状存在较大的遗传变异。耐盐品种CM 72在200 mM NaCl处理下的气孔开度显著大于盐敏感品种Gairkanov。盐胁迫下气孔宽/长等性状与籽粒产量呈显著正相关。通过对CM 72/Gairlaud的分离双单倍体群体的表型特征和QTL分析，鉴定出了与气孔性状相关的显著的耐盐性QTL。此外，对慢阴离子通道基因HvSLAH 1和HvSLAC 1的表达分析表明，它们的上调与田间大麦籽粒产量的提高有关。
Soil salinity is an environmental and agricultural problem in many parts of the world. One of the keys to breeding barley for adaptation to salinity lies in a better understanding of the genetic control of stomatal regulation. We have employed a range of physiological (stomata assay, gas exchange, phylogenetic analysis, QTL analysis), and molecular techniques (RT-PCR and qPCR) to investigate stomatal behavior and genotypic variation in barley cultivars and a genetic population in four experimental trials. A set of relatively efficient and reliable methods were developed for the characterization of stomatal behavior of a large number of varieties and genetic lines. Furthermore, we found a large genetic variation of gas exchange and stomatal traits in barley in response to salinity stress. Salt-tolerant cultivar CM72 showed significantly larger stomatal aperture under 200 mM NaCl treatment than that of salt-sensitive cultivar Gairdner. Stomatal traits such as aperture width/length were found to significantly correlate with grain yield under salt treatment. Phenotypic characterization and QTL analysis of a segregating double haploid population of the CM72/Gairdner resulted in the identification of significant stomatal traits-related QTLs for salt tolerance. Moreover, expression analysis of the slow anion channel genes HvSLAH1 and HvSLAC1 demonstrated that their up-regulation is linked to higher barley grain yield in the field.
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DOI：
10.1073/pnas.1215265109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Dai, Fei;Nevo, Eviatar;Zhang, Guoping
通讯作者：
Zhang, Guoping
转录组分析揭示了现代栽培大麦的镶嵌基因组起源
DOI：
10.1073/pnas.1414335111
发表时间：
2014-09-16
影响因子：
11.1
作者：
Dai, Fei;Chen, Zhong-Hua;Zhang, Guoping
通讯作者：
Zhang, Guoping
影响因子：
6.9
作者：
Chen, Zhonghua;Cuin, Tracey A.;Shiabala, Sergey
通讯作者：
Shiabala, Sergey
影响因子：
6.9
作者：
EDWARDS, M;MEIDNER, H
通讯作者：
MEIDNER, H
影响因子：
11.6
作者：
Maierhofer, Tobias;Lind, Christof;Geiger, Dietmar
通讯作者：
Geiger, Dietmar