Lipopolysaccharide Exhibits Synergistic Enhancement of Butyrate-Induced and Retinoic Acid-Mediated Alkaline Phosphatase Activity on Small Intestinal Epithelial Cell Line, IEC-6
Lipopolysaccharide Exhibits Synergistic Enhancement of Butyrate-Induced and Retinoic Acid-Mediated Alkaline Phosphatase Activity on Small Intestinal Epithelial Cell Line, IEC-6
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脂多糖对小肠上皮细胞系 IEC-6 表现出丁酸盐诱导和视黄酸介导的碱性磷酸酶活性的协同增强作用
作者：
K. Fukushima;I. Sasaki;Kenichi Takahashi;H. Naito;Hitoshi Ogawa;Shun Sato;S. Matsuno
虽然脂多糖（LPS）和丁酸钠是主要的细菌产物和生物活性化学物质，对肠道粘膜细胞具有多种功能，但它们对上皮细胞的相互作用还不清楚。本研究的目的是调查是否脂多糖调制丁酸诱导和视黄酸介导的碱性磷酸酶（ALP）的IEC-6细胞-大鼠非转化小肠上皮细胞系的活性。当细胞达到汇合时，向培养物中加入丁酸钠、视黄酸和LPS的各种组合。然后收获细胞用于测量ALP活性。丁酸钠，而不是单独的视黄酸或LPS，增强ALP活性。当LPS与丁酸或视黄酸一起使用时，证实了ALP活性的协同诱导。当胞壁酰肽或N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸与这些酸一起使用时，未观察到ALP活性的累加效应。本研究清楚地表明，丁酸盐和LPS的特定组合协同增加肠上皮细胞系的ALP活性，这是一种上皮分化相关的标志物。
Although both lipopolysaccharide (LPS) and sodium butyrate are major bacterial products and bioactive chemicals with multiple functions on mucosal cells in the gut, their interaction effects on epithelial cells are not well understood. The purpose of the present study was to investigate whether LPS modulates butyrate-induced and retinoic acid-mediated alkaline phosphatase (ALP) activity of IEC-6 cells – a rat nontransformed small intestinal epithelial cell line. When cells reached confluency, various combinations of sodium butyrate, retinoic acid and LPS were added to the cultures. Cells were then harvested for the measurement of ALP activities. Sodium butyrate, but not retinoic acid or LPS alone, enhanced ALP activity. When LPS was additionally used with butyrate or retinoic acid, synergistic induction of ALP activities was demonstrated. No additive effect for ALP activity was observed when muramyl peptides or N-formylmethionyl-leucyl-phenylalanine was used with these acids. The present study clearly demonstrated that the specific combination of butyrate and LPS synergistically increased ALP activity, an epithelial differentiation-associated marker, of an intestinal epithelial cell line.
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作者：
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DOI：
--
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DOI：
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