A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments.
A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments.
复制标题
一种由热塑性面罩制成的新型兔子固定器，用于清醒成像实验
DOI：
10.1038/s41598-021-81358-6
复制
发表时间：
2021-01-15
影响因子：
4.6
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Lu R;Hou L;Wang S;She B;He H;Gao W;Wang S;Xv D;Ji Y;Yang S;Yang Z;Wang S
本研究旨在开发和验证一种由热塑性面罩制成的用于清醒成像实验的新型兔固定器。当在65-70 °C的热水浴中加热2-5分钟时，热塑性面罩变软，并且可以模制以适合麻醉兔的整个身体（通过肌内注射4 ml的3%戊巴比妥钠溶液）。20只家兔随机分为固定组和麻醉组，每组10只。测量并比较动物的生命体征、应激激素（皮质醇和肾上腺素）以及计算机断层扫描（CT）、正电子发射断层扫描（PET）和磁共振成像（MRI）扫描的主观图像质量评分。通过使用不同浓度或不同放射性的成像剂进行体模CT、MRI和PET研究，以评估有和没有热塑性口罩的性能。麻醉后呼吸频率（RR）、收缩压（SBP）、舒张压（DBP）、外周毛细血管血氧饱和度（SpO 2）、体温（T）均下降（P均< 0.05），固定后无明显下降（P均> 0.05）。心率、皮质醇和肾上腺素在麻醉和固定后均无明显下降（P > 0.05）。头、胸、肝、肾、肠、骨盆CT和MRI图像的主观图像质量评分以及PET图像的主观图像质量评分在两组之间无显著性差异（均P > 0.05）。除肌肉外，其他脏器18F-FDG代谢均低于麻醉后，肝脏18F-FDG代谢与麻醉后无明显差异。体模研究显示，有无面罩的CT值、标准摄取值和MR T2信号值无显著性差异（均P > 0.05）。使用热塑性掩模创建的新型兔固定器可用于在清醒和接近生理状态下获得不同成像方式的高质量图像。
This study aimed to develop and validate a novel rabbit fixator made from a thermoplastic mask for awake imaging experiments. When heated in a hot-water bath at 65–70 °C for 2–5 min, the thermoplastic mask became soft and could be molded to fit over the entire body of an anesthetized rabbit (4 ml of 3% pentobarbital sodium solution by intramuscular injection). Twenty rabbits were randomly divided into fixator (n = 10) and anesthesia (n = 10) groups. The animals’ vital signs, stress hormones (cortisol and adrenaline), and subjective image quality scores for the computed tomography (CT), positron emission tomography (PET), and magnetic resonance imaging (MRI) scanning were measured and compared. Phantom CT, MRI and PET studies were performed to assess the performance with and without the thermoplastic mask by using image agents at different concentrations or with different radioactivity. The respiration rate (RR), systolic blood pressure (SBP), diastolic blood pressure (DBP), peripheral capillary oxygen saturation (SpO2) and body temperature (T) decreased after anesthesia (all P < 0.05) but did not significantly decrease after fixation (all P > 0.05). The heart rate (HR), cortisol and adrenaline did not significantly decrease after either anesthesia or fixation (all P > 0.05). The subjective image quality scores for the CT and MRI images of the head, thorax, liver, kidney, intestines and pelvis and the subjective image quality scores for the PET images did not significantly differ between the two groups (all P > 0.05). For all examined organs except the muscle, 18F-FDG metabolism was lower after fixation than after anesthesia, and was almost identical of liver between two groups. The phantom study showed that the CT values, standard uptake values and MR T2 signal values did not differ significantly with or without the mask (all P > 0.05). A novel rabbit fixator created using a thermoplastic mask could be used to obtain high-quality images for different imaging modalities in an awake and near-physiological state.
登录
查看更多内容
影响因子：
3
作者：
Drucker CB;Carlson ML;Toda K;DeWind NK;Platt ML
通讯作者：
Platt ML
DOI：
10.30802/aalas-jaalas-18-000078
发表时间：
2019-03-01
影响因子：
1.7
作者：
Bradley, Michael P.;Doerning, Carolyn M.;Lester, Patrick A.
通讯作者：
Lester, Patrick A.
影响因子：
5.7
作者：
Hideghety, Katalin;Cserhati, Adrienn;Thurzo, Laszlo
通讯作者：
Thurzo, Laszlo
DOI：
10.1186/s13014-015-0484-7
发表时间：
2015-08-20
期刊：
Radiation oncology (London, England)
影响因子：
--
作者：
Navarro-Martin A;Cacicedo J;Leaman O;Sancho I;García E;Navarro V;Guedea F
通讯作者：
Guedea F
影响因子：
5.7
作者：
Fuss, M;Salter, BJ;Herman, TS
通讯作者：
Herman, TS