Upregulation of sialyltransferases ST3Gal1 and ST6Gal1 promotes stabilization of erythrocyte mass and recovery of anemia in Trypanosoma brucei brucei-infected pigs.
Upregulation of sialyltransferases ST3Gal1 and ST6Gal1 promotes stabilization of erythrocyte mass and recovery of anemia in Trypanosoma brucei brucei-infected pigs.
复制标题
唾液酸转移酶ST3Gal 1和ST6Gal 1的上调促进布氏锥虫感染猪的红细胞质量稳定和贫血恢复。
DOI：
10.1016/j.rvsc.2022.02.012
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发表时间：
2022-07
影响因子：
2.4
通讯作者：
Esievo, K. A. N.
中科院分区：
文献类型：
--
作者：
Atata, J. A.;Enam, S. J.;Ogbuagu, N. E.;Balogun, E. O.;Adamu, S.;Esievo, K. A. N.
研究了唾液酸转移酶（STs）特异性亚家族ST 3Gal 1和ST 6 Gal 1在布氏锥虫感染猪和未感染对照猪的肝脏和肾脏中的组织表达的作用。该研究旨在为治疗提供新的靶点。用2 × 106 T. B.布氏杆菌（Federe株）;通过显微镜监测寄生虫血症，并使用定量实时聚合酶链反应（qRT-PCR）评估肝脏和肾脏中ST 3Gall和ST 6 Gall的组织表达水平。感染猪的寄生虫血症呈波动性，在第13天显著发生贫血（P < 0.01），并试图在第21天恢复至研究终止。与未感染的对照组相比，在感染的猪中肝和肾ST 3Gal 1和ST 6 Gal 1的基因表达显著（P < 0.0001）上调5-42倍。从本研究中的发现得出结论，在T B中增加ST 3 Gal 1和ST 6 Gal 1的组织表达。布鲁氏菌感染的猪可能在去唾液酸化红细胞的再唾液酸化中起关键作用，从而促进锥虫感染的猪中红细胞的恢复和红细胞质量的稳定。建议进一步研究血清ST 3Gal 1和ST 6 Gal 1在锥虫易感动物和锥虫耐受动物中的表达，以确定这些基因在锥虫耐受中的作用。
The role of Sialyltransferases (STs) specifically subfamilies ST3Gal1 and ST6Gal1 tissue expression was investigated in the liver and kidney of Trypanosoma brucei brucei-infected and uninfected control pigs. The study was aimed to provide emerging target for treatment. Pigs were experimentally infected with 2 × 106 T. b. brucei (Federe strain); parasitemia was monitored by microscopy and tissue expression levels of ST3Gall and ST6Gall in the liver and kidney were assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Parasitemia were undulating and anemia occurred significantly (P < 0.01) on day 13 in the infected pigs with an attempt to recover toward the termination of the study on day 21. The gene expressions for hepatic and renal ST3Gal1 and ST6Gal1 were significantly (P < 0.0001) upregulated 5–42 folds in the infected pig compared to the non-infected control group. It was concluded from the findings in this study that increased tissue expression of ST3Gal1 and ST6Gal1 in T b. brucei-infected pigs may play a pivotal role in the resialylation of desialylated red blood cells, thereby promoting recovery of the red blood cells and stabilization of erythrocyte mass in trypanosome-infected pigs. It is recommended that the expression of serum ST3Gal1 and ST6Gal1 be investigated further, in trypano-susceptible against trypano-tolerant breeds of animals to determine the role of these genes in trypano-tolerance.
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影响因子：
1.2
作者：
Bork, Kaya;Weidemann, Wenke;Horstkorte, Ruediger
通讯作者：
Horstkorte, Ruediger
影响因子：
2.7
作者：
Nok, AJ;Balogun, EO
通讯作者：
Balogun, EO
影响因子：
--
作者：
Hsieh CC;Shyr YM;Liao WY;Chen TH;Wang SE;Lu PC;Lin PY;Chen YB;Mao WY;Han HY;Hsiao M;Yang WB;Li WS;Sher YP;Shen CN
通讯作者：
Shen CN
影响因子：
3.3
作者：
Cohen, Miriam;Varki, Ajit
通讯作者：
Varki, Ajit
影响因子：
3.4
作者：
Ou, Lingling;He, Xiuzhen;Lin, Shaoqiang
通讯作者：
Lin, Shaoqiang