Bone marrow mesenchymal stem cells increase motility of prostate cancer cells via production of stromal cell-derived factor-1α.
Bone marrow mesenchymal stem cells increase motility of prostate cancer cells via production of stromal cell-derived factor-1α.
复制标题
DOI：
10.1111/jcmm.12010
复制
发表时间：
2013-02
影响因子：
5.3
通讯作者：
Penna C
中科院分区：
文献类型：
--
作者：
Mognetti B;La Montagna G;Perrelli MG;Pagliaro P;Penna C
前列腺癌经常转移到骨，癌细胞和骨微环境之间的相互作用已被证明是建立新的转移的关键。骨髓间充质干细胞（BM-MSCs）分泌多种细胞因子，调节周围细胞的行为。然而，关于BM-MSCs在影响前列腺癌细胞迁移和侵袭中的作用知之甚少。我们推测BM-MSCs释放的基质细胞衍生因子-1 α可能在这些过程中发挥关键作用。为了研究骨髓间充质干细胞分泌的因子与前列腺癌细胞的相互作用，我们建立了骨髓间充质干细胞与前列腺癌细胞DU 145的体外transwell共培养模型。使用这个模型，我们已经表明BM-MSC产生可溶性因子，其增加前列腺癌细胞DU 145的运动性。通过阻断抗体中和基质细胞衍生因子-1 α（SDF 1 α）可显著限制骨髓MSC的化学吸引作用。此外，BM-MSC产生的可溶性因子极大地激活促存活激酶，即AKT和ERK 1/2。我们提供了进一步的证据表明SDF 1 α参与前列腺癌细胞和BM-MSCs之间的相互作用。这种相互作用可能在前列腺癌细胞在骨内的迁移和侵袭中起重要作用。
Prostate cancer frequently metastasizes to the bone, and the interaction between cancer cells and bone microenvironment has proven to be crucial in the establishment of new metastases. Bone marrow mesenchymal stem cells (BM-MSCs) secrete various cytokines that can regulate the behaviour of neighbouring cell. However, little is known about the role of BM-MSCs in influencing the migration and the invasion of prostate cancer cells. We hypothesize that the stromal cell-derived factor-1α released by BM-MSCs may play a pivotal role in these processes. To study the interaction between factors secreted by BM-MSCs and prostate cancer cells we established an in vitro model of transwell co-culture of BM-MSCs and prostate cancer cells DU145. Using this model, we have shown that BM-MSCs produce soluble factors which increase the motility of prostate cancer cells DU145. Neutralization of stromal cell-derived factor-1α (SDF1α) via a blocking antibody significantly limits the chemoattractive effect of bone marrow MSCs. Moreover, soluble factors produced by BM-MSCs greatly activate prosurvival kinases, namely AKT and ERK 1/2. We provide further evidence that SDF1α is involved in the interaction between prostate cancer cells and BM-MSCs. Such interaction may play an important role in the migration and the invasion of prostate cancer cells within bone.
登录
查看更多内容
影响因子：
11.2
作者：
Liu S;Ginestier C;Ou SJ;Clouthier SG;Patel SH;Monville F;Korkaya H;Heath A;Dutcher J;Kleer CG;Jung Y;Dontu G;Taichman R;Wicha MS
通讯作者：
Wicha MS
影响因子：
2.4
作者：
Raimondo, S;Penna, C;Geuna, S
通讯作者：
Geuna, S
影响因子：
3.3
作者：
Ok, Sooho;Kim, Sung-Moo;Ahn, Kwang Seok
通讯作者：
Ahn, Kwang Seok
影响因子：
6.2
作者：
Cooper, CR;Chay, CH;Pienta, KJ
通讯作者：
Pienta, KJ
影响因子：
3.8
作者：
Schiller KR;Zillhardt MR;Alley J;Borjesson DL;Beitz AJ;Mauro LJ
通讯作者：
Mauro LJ