MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases.
MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases.
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MET 过表达粘液纤维肉瘤经常表现出 7 号染色体多体性，但不表现出 MET 扩增，尤其是在高级别病例中：30 例粘液纤维肉瘤病例的临床和病理回顾
DOI：
10.1186/s13000-018-0733-9
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发表时间：
2018-08-21
影响因子：
2.6
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Ma S;Fan L;Liu Y;Wang Y;Yu K;Wang L;Fang N;Liu F;Guo S;Wang Z
背景粘纤维肉瘤（MFS）是最常见的软组织肉瘤之一。先前的研究表明，MET蛋白在MFS患者中过度表达，可以作为预后因素。 MET 蛋白过度表达的原因包括 MET 基因的扩增，该基因位于染色体 7q。以黏液纤维肉瘤中存在7号染色体多体性而非MET基因扩增的指示病例为触发点，我们对更多病例进行了7号染色体多体性的研究。方法对30例MFS（包括2例上皮样变异）进行免疫组织化学和荧光原位杂交（FISH）检测MET蛋白表达和基因状态。结果30例中14例MET过表达，13例MET过表达。病例处于较高的 FNCLCC 等级（2-3 级）。 FISH显示11例Met平均有3个信号，着丝粒7q（CEP7q）有3个以上信号（平均值：4.6）。 MET/CEP7比值平均约为0.65，表明7号染色体多体性，而不是Met基因扩增，导致MFS中MET蛋白的过度表达。 MET过表达和7号染色体多体性与较高的Ki-67指数和较高的分级呈正相关，可能具有较高的局部复发和转移风险。结论可能揭示粘纤维肉瘤中MET过表达的另一种解释，为MFS的治疗提供线索。
BackgroundMyxofibrosarcoma (MFS) is one of the most common soft tissue sarcomas. Previous studies have shown that MET protein overexpressed in MFS patients and can serve as a prognostic factor. The reasons for MET protein overexpression include amplification of the MET gene, which is located on chromosome 7q. Triggered by an index case harboring chromosome 7 polysomy rather than MET gene amplification in myxofibrosarcoma, we investigated chromosome 7 polysomy in more cases.MethodsImmunohistochemistry and fluorescence in situ hybridization (FISH) were performed in 30 MFS cases (including 2 epithelioid variant) to detect the expression of MET protein and gene status.ResultsMET was overexpressed in 14 cases out of 30, while thirteen cases were in higher FNCLCC grades (Grade 2–3). FISH showed that 11 cases having 3 signals on average ofMetand more than 3 signals (Mean: 4.6) of centromere 7q (CEP7q). The MET/CEP7 ratio was about 0.65 on average, suggesting that chromosome 7 polysomy, rather thanMetgene amplification, leading to the overexpression of MET protein in MFS. MET overexpression and chromosome 7 polysomy are positively correlated with higher Ki-67 index and higher grade and might have a high risk of local recurrence and metastasis.ConclusionsIt might reveals another explain of MET overexpression in myxofibrosarcoma, providing a clue for the therapy of MFS.
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MET蛋白过表达和基因扩增在局部晚期鼻咽癌中的预后价值
影响因子：
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作者：
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Huang, Hsuan-Ying
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作者：
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通讯作者：
Matsubara, Osamu
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