Micro-light-pipe array with an excitation attenuation filter for lensless digital enzyme-linked immunosorbent assay
Micro-light-pipe array with an excitation attenuation filter for lensless digital enzyme-linked immunosorbent assay
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用于无透镜数字酶联免疫吸附测定的具有激发衰减滤波器的微光管阵列
DOI：
10.7567/jjap.55.03df03
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发表时间：
2016
影响因子：
1.5
通讯作者：
J. Ohta
中科院分区：
文献类型：
--
作者：
H. Takehara;M. Nagasaki;K. Sasagawa;H. Takehara;T. Noda;T. Tokuda;J. Ohta
数字化酶联免疫吸附试验(EL ISA)用于检测各种具有超敏反应的生物标志物。我们一直在开发紧凑型系统，用cmos图像传感器取代荧光显微镜。在这里，我们提出了一种由金属填充染料掺杂树脂制成的微光管阵列结构，可以作为数字ELISA微反应室阵列的制作衬底。用一个简单的模型模拟了这种结构提高荧光耦合效率的可能性。为了实现这种结构，我们在厚的光刻胶图形周围通过电镀铜的方法制备了30微米厚的微管阵列。每根微管的典型直径为10微米，管内填充了黄色染料掺杂的环氧树脂。通过调节掺杂浓度，可以控制荧光和激发光的透过率。我们证实了角度激发光的入射抑制了激发光的泄漏。
Digital enzyme-linked immunosorbent assay (ELISA) is used for detecting various biomarkers with hypersensitivity. We have been developing compact systems by replacing the fluorescence microscope with a CMOS image sensor. Here, we propose a micro-light-pipe array structure made of metal filled with dye-doped resin, which can be used as a fabrication substrate of the micro-reaction-chamber array of digital ELISA. The possibility that this structure enhances the coupling efficiency for fluorescence was simulated using a simple model. To realize the structure, we fabricated a 30-µm-thick micropipe array by copper electroplating around a thick photoresist pattern. The typical diameter of each fabricated micropipe was 10 µm. The pipes were filled with yellow-dye-doped epoxy resin. The transmittance ratio of fluorescence and excitation light could be controlled by adjusting the doping concentration. We confirmed that an angled excitation light incidence suppressed the leakage of excitation light.
影响因子：
8.4
作者：
Daivasagaya, Daisy S.;Yao, Lei;Yung, Ka Yi;Hajj-Hassan, Mohamad;Cheung, Maurice C.;Chodavarapu, Vamsy P.;Bright, Frank V.
通讯作者：
Bright, Frank V.