Alterations in chromatin accessibility during osteoblast and adipocyte differentiation in human mesenchymal stem cells.
Alterations in chromatin accessibility during osteoblast and adipocyte differentiation in human mesenchymal stem cells.
复制标题
人间充质干细胞成骨细胞和脂肪细胞分化过程中染色质可及性的变化
DOI：
10.1186/s12920-022-01168-1
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发表时间：
2022-01-31
影响因子：
2.7
通讯作者：
Xiong J
中科院分区：
文献类型：
--
作者：
Liu J;Gan L;Ma B;He S;Wu P;Li H;Xiong J
虽然在骨髓间充质干细胞（MSCs）的成脂/成骨分化过程中基因的差异表达是明显的，但尚不清楚这是否与染色体结构的变化有关。在这项研究中，我们使用atac测序技术观察了MSC分化早期染色质组装的变化。这表明在成脂/成骨分化的不同时间点染色体可接近性的数量和分布发生了显著变化。差异峰的测序表明参与MSC分化的转录因子基序发生了变化。基因本体（Gene Ontology， GO）和通路分析表明，染色质可及性的改变导致了生物学功能的改变。然后我们整合了ATAC-seq和RNA-seq，发现只有一小部分重叠基因从ATAC-seq和RNA-seq重叠中被筛选出来。通过氧化石墨烯和对这些重叠基因的通路分析，我们不仅观察到一些已知的与成脂/成骨分化相关的生物学功能，还注意到MSC分化过程中一些不寻常的生物聚类。总之，我们的工作不仅展示了MSC在分化过程中染色质可及性的景观，而且有助于进一步理解这些过程中基因表达的潜在机制。
Although differential expression of genes is apparent during the adipogenic/osteogenic differentiation of marrow mesenchymal stem cells (MSCs), it is not known whether this is associated with changes in chromosomal structure. In this study, we used ATAC-sequencing technology to observe variations in chromatin assembly during the early stages of MSC differentiation. This showed significant changes in the number and distribution of chromosome accessibility at different time points of adipogenic/osteogenic differentiation. Sequencing of differential peaks indicated alterations in transcription factor motifs involved in MSC differentiation. Gene Ontology (GO) and pathway analysis indicated that changes in biological function resulted from the alterations in chromatin accessibility. We then integrated ATAC-seq and RNA-seq and found that only a small proportion of the overlapping genes were screened out from ATAC-seq and RNA-seq overlapping. Through GO and pathway analysis of these overlapped genes, we not only observed some known biological functions related to adipogenic/osteogenic differentiation but also noticed some unusual biological clustering during MSC differentiation. In summary, our work not only presents the landscape of chromatin accessibility of MSC during differentiation but also helps to further our understanding of the underlying mechanisms of gene expression in these processes.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
10.7
作者：
Amoutzias, G. D.;Veron, A. S.;Robertson, D. L.
通讯作者：
Robertson, D. L.
影响因子：
4.3
作者：
Bowers, Robert R.;Lane, M. Daniel
通讯作者：
Lane, M. Daniel
影响因子：
6.2
作者：
Qiu, Weimin;Andersen, Tom E.;Kassem, Moustapha
通讯作者：
Kassem, Moustapha
暴露于地塞米松的人类成骨细胞中的基因表达分析鉴定出改变的发育途径是骨质疏松的推定驱动因素。
影响因子：
2.3
作者：
Hurson CJ;Butler JS;Keating DT;Murray DW;Sadlier DM;O'Byrne JM;Doran PP
通讯作者：
Doran PP