Dithiothreitol, a disulfide‐reducing agent, inhibits capacitation, acrosome reaction, and interaction with eggs by guinea pig spermatozoa
Dithiothreitol, a disulfide‐reducing agent, inhibits capacitation, acrosome reaction, and interaction with eggs by guinea pig spermatozoa
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二硫苏糖醇是一种二硫化物还原剂，可抑制豚鼠精子的获能、顶体反应以及与卵子的相互作用
DOI：
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
G. Nicolson
中科院分区：
文献类型：
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作者：
R. Yanagimachi;Thomas T. F. Huang;A. D. Fleming;N. Kosower;G. Nicolson
二硫还原剂二硫苏糖醇（DTT）可抑制豚鼠精子体外获能。即使是短暂的治疗DTT抑制获能，除非氧化剂（谷胱甘肽二硫化物）存在于后处理介质中。预获能的精子在DTT存在下不能进行顶体反应，表明该试剂也阻断顶体反应。顶体反应的精子在DTT存在下不能附着和穿透透明膜。即使当顶体反应的精子被直接带到无透明带的卵子的表面时，只要DTT存在于培养基中，它们就不能与卵子质膜结合并融合。这些观察结果表明，三级和四级结构的精子表面蛋白的巯基二硫化物状态的调节是至关重要的精子的生理和功能的初步受精过程中。
Capacitation of guinea pig spermatozoa in vitro was inhibited by the disulfide-reducing agent dithiothreitol (DTT). Even a brief treatment with DTT inhibited capacitation unless an oxidizing agent (glutathione disulfide) was present in the posttreatment medium. Precapacitated spermatozoa were unable to undergo the acrosome reaction in the presence of DTT, indicating that this reagent also blocks the acrosome reaction. Acrosome-reacted spermatozoa were incapable of attaching to and penetrating the zona pellucida in the presence of DTT. Even when acrosome-reacted spermatozoa were directly brought to the surface of zona-free eggs, they were unable to bind to and fuse with the egg plasma membrane so long as DTT was present in the medium. These observations suggest that the tertiary and quaternary structures of sperm surface proteins regulated by their thioldisulfide status are of critical importance in the physiology and function of spermatozoa preliminary to and in the process of fertilization.
胰岛素受体：脂肪细胞和肝质膜上结构组织的差异。
DOI：
10.1126/science.7003710
发表时间：
1980
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jarett,L;Schweitzer,JB;Smith,RM
通讯作者：
Smith,RM