Oxidative stress impairs the calcification ability of human dental pulp cells.
Oxidative stress impairs the calcification ability of human dental pulp cells.
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DOI：
10.1186/s12903-022-02467-w
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发表时间：
2022-10-03
期刊：
影响因子：
2.9
通讯作者：
Shiba, Hideki
中科院分区：
文献类型：
--
作者：
Shirawachi, Satomi;Takeda, Katsuhiro;Naruse, Tomoya;Takahasi, Yohei;Nakanishi, Jun;Shindo, Satoru;Shiba, Hideki
内根吸收和氧化应激之间的关系尚未报道。本研究的目的是增加对内根吸收的分子认识。本研究旨在探讨过氧化氢（H2 O2）作为氧化应激诱导剂对人牙髓细胞（hDPC）钙化能力的影响及三磷酸肌醇（IP 3）的参与作用。hDPC（Lonza，巴塞尔，瑞士）暴露于H2 O2。然后评价细胞活力和活性氧（ROS）产生。为探讨H2 O2对hDPC钙化能力的影响，采用实时荧光定量PCR、碱性磷酸酶（ALP）染色和茜素红染色方法检测hDPC碱性磷酸酶（ALP）mRNA表达。将数据与用2-氨基乙基二苯基硼酸盐（2-APB）预处理的hDPC的数据进行比较，2-APB是IP 3受体抑制剂。浓度高于250 µM的H2 O2显著降低细胞活力（P < 0.01）。100 μM H2 O2处理的hDPC产生的ROS比对照细胞多（P < 0.01）。2-APB显著降低产蛋率（P < 0.05）。与阴性对照组相比，H2 O2处理的hDPC ALP mRNA表达（P < 0.01）、ALP活性（P < 0.01）和矿化结节沉积（P < 0.01）均显著降低。2-APB可明显抑制上述降低（P < 0.01、P < 0.05和P < 0.01）。数据代表三个独立实验，每个处理重复三次，值表示为平均值± SD。据我们所知，这是第一个研究记录IP 3信号参与H2 O2损害的人牙髓细胞钙化能力。
The relationship between internal root resorption and oxidative stress has not yet been reported. This study aimed to add molecular insight into internal root resorption. The present study was conducted to investigate the effect of hydrogen peroxide (H2O2) as an inducer of oxidative stress on the calcification ability of human dental pulp cells (hDPCs) and the involvement of inositol 1, 4, 5-trisphosphate (IP3). hDPCs (Lonza, Basel, Switzerland) were exposed to H2O2. Cell viability and reactive oxygen species (ROS) production were then evaluated. To investigate the effect of H2O2 on the calcification ability of hDPCs, real-time PCR for alkaline phosphatase (ALP) mRNA expression, ALP staining, and Alizarin red staining were performed. Data were compared with those of hDPCs pretreated with 2-aminoethyldiphenylborate (2-APB), which is an IP3 receptor inhibitor. H2O2 at concentrations above 250 µM significantly reduced cell viability (P < 0.01). More ROS production occurred in 100 µM H2O2-treated hDPCs than in control cells (P < 0.01). 2-APB significantly decreased the production (P < 0.05). H2O2-treated hDPCs showed significant reductions in ALP mRNA expression (P < 0.01), ALP activity (P < 0.01), and mineralized nodule deposition compared with negative control cells (P < 0.01). 2-APB significantly inhibited these reductions (P < 0.01, P < 0.05 and P < 0.01, respectively). Data are representative of three independent experiments with three replicates for each treatment and values are expressed as means ± SD. To the best of our knowledge, this is the first study documenting the involvement of IP3 signaling in the calcification ability of human dental pulp cells impaired by H2O2.
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