Bombesin Receptors on Gastrin Cells a
Bombesin Receptors on Gastrin Cells a
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胃泌素细胞上的铃蟾肽受体
DOI：
--
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发表时间：
1988
影响因子：
5.2
通讯作者：
P. Mantyh
中科院分区：
文献类型：
--
作者：
S. Vigna;A. Giraud;A. Soll;J. Walsh;P. Mantyh
正如本卷其他部分详细讨论的，铃蟾肽是一种两栖动物皮肤肽，对哺乳动物的中枢神经系统和外周组织具有广泛的生物作用。铃蟾肽的哺乳动物对应物称为胃泌素释放肽（GRP），因为响应于猪胃提取物的胃泌素释放被用作从此类提取物中纯化猪 GRP 的活性测定。当猪 GRP 被分离并测序时，2 发现它在化学和生物学上与两栖动物铃蟾肽同源。 G R P 的羧基端九肽序列与铃蟾肽的羧基端九肽序列相同，只是在羧基端第8位用组氨酸取代了谷氨酰胺残基。已经证明，铃蟾肽的羧基末端九肽序列是完整生物活性所必需的。具有可测量生物活性的最小的ombePin片段是羧基端七肽，羧基端八肽的效力介于hepa和九肽之间。因此，铃蟾肽和 GRP 的羧基末端区域在进化中高度保守也就不足为奇了，因为该区域是该调节肽家族中生物活性最重要的决定因素。尽管铃蟾肽和 GRP 在哺乳动物中的识别作用是刺激胃泌素的释放，但人们对这种作用的机制知之甚少。铃蟾肽可刺激狗、大鼠和人类体内的胃泌素分泌！然而，铃蟾肽和 GRP 释放胃泌素的作用位点尚未在任何物种中得到最终证实。分析调节物质作用机制的首要要求之一是识别靶细胞。间接证据表明铃蟾肽和 GRP 对胃窦胃泌素细胞有直接作用，但尚无明确的证据证明这一点。当给予铃蟾肽时，分离的、灌注的大鼠胃已被证明会释放胃泌素进入循环系统，但也会释放生长抑素。”铃蟾肽在体外引起大鼠胃窦腺释放胃泌素，但不释放生长抑素。9最后，富含胃泌素细胞但也含有其他粘膜细胞类型的犬窦粘膜细胞制剂最近已被证明可响应铃蟾肽将胃泌素释放到培养基中。10*”因此，焦点已逐渐从整个动物缩小到分离的胃，再到分离的胃
As discussed in detail elsewhere in this volume, bombesin is an amphibian skin peptide with a wide spectrum of biological actions on the central nervous system and on peripheral tissues in mammals. The mammalian counterpart of bombesin is called gastrin-releasing peptide (GRP), because gastrin release in response to potcine gastric extracts was used as the assay of activity in the purification of porcine GRP from such extracts.' When porcine G R P was isolated and sequenced,2 it was found to be chemically as well as biologically homologous to amphibian bombesin. The carboxylterminal nonapeptide sequence of G R P is identical to that of bombesin except for the single substitution of a histidine for a glutamine residue at position 8 from the carboxyl terminus. It has been demonstrated that the carboxyl-terminal nonapeptkle sequence of bombesin is required for full biological activity.' The minimum bombePin fragment with measurable biological activity is the carboxyl-terminal heptapeptider the carboxyl-terminal octapeptide is intermediate in potency between the hepaand nonapeptide.' It is not surprising then that the carboxyl-terminal region of bombesin and of GRP have been highly conserved in evolution, since this region is the most important determinant of biological activity in this family of regulatory peptides. Although the identifying action of bombesin and G R P in mammals is stimulation of the release of gastrin, little is known about the mechanism of this actian. Bombesin stimulates gastrin secretion in vivo in dogs: rats,' and humans! However, the site of action of bombesin and GRP to release gastrin has not been conclusively demonstrated in any species. One of the first requirements for analysis of the mechanism of action of regulatory substances is to identify the target cell. Indirect evidence suggests a direct action of bombesin and G R P on antral gastrin cells, but there has been no unequivocal demonstration of this. Isolated, perfused rat stomachs have been shown to release gastrin into the circulation when bombesin is administered, but somatostatin is also released.'.' Bombesin causes release of gastrin, but not somatostatin, from rat antral glands in vitro.9 Finally, preparations of canine antral mucosal cells enriched in gastrin cells, but also containing other mucosal cell types, have recently been shown to release gastrin into the culture medium in response to bombesin.lO*" Thus, the focus has been increasingly narrowed from whole animals down to isolated stomachs, to isolated
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DOI：
10.1016/s0021-9258(18)32209-9
发表时间：
1983-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Westendorf;A. Schonbrunn
通讯作者：
J. Westendorf;A. Schonbrunn
犬胃肠道中铃蟾肽特异性结合位点的定位。
影响因子：
29.4
作者：
Vigna,SR;Mantyh,CR;Giraud,AS;Soll,AH;Walsh,JH;Mantyh,PW
通讯作者：
Mantyh,PW
离体灌注大鼠胃胃泌素和生长抑素分泌的差异调节。
影响因子：
29.4
作者：
Martindale,R;Kauffman,GL;Levin,S;Walsh,JH;Yamada,T
通讯作者：
Yamada,T
铃蟾肽和犬胃泌素释放肽刺激胃泌素释放。
DOI：
10.1172/jci112904
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Sugano,K;Park,J;Soll,AH;Yamada,T
通讯作者：
Yamada,T