Instillation of six different ultrafine carbon particles indicates a surface area threshold dose for acute lung inflammation in mice.
Instillation of six different ultrafine carbon particles indicates a surface area threshold dose for acute lung inflammation in mice.
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DOI：
10.1289/ehp.8266
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发表时间：
2006-03
影响因子：
10.4
通讯作者：
Schulz H
中科院分区：
文献类型：
--
作者：
Stoeger T;Reinhard C;Takenaka S;Schroeppel A;Karg E;Ritter B;Heyder J;Schulz H
空气颗粒物污染水平的增加与呼吸系统和心血管疾病死亡率和发病率的增加有关。一些流行病学和毒理学研究表明，单位质量的超细颗粒 (UFP)（< 100 nm）比较大颗粒的危害更大。我们的研究旨在定量比较不同类型碳质 UFP 在引起肺部中度炎症反应的剂量范围内的急性不良反应。我们使用了六种不同的颗粒类型（初级颗粒尺寸 10–50 nm，比表面积 30–800 m2/g，有机物含量 1–20%）：PrintexG、Printex90、具有不同有机物含量的火焰烟灰颗粒（SootL、SootH）、火花产生的超细碳颗粒（ufCP）和参考柴油机尾气颗粒（DEP）SRM1650a。向小鼠滴注每种颗粒类型 5、20 和 50 μg，并在滴注 24 小时后分析支气管肺泡灌洗液中的炎症细胞和促炎细胞因子的水平。在各自的质量剂量下，颗粒引起的有害影响按以下顺序排列：ufCP > SootL ≥ SootH > Printex90 > PrintexG > DEP。将炎症效应与颗粒特征（有机含量、初级颗粒大小或比表面积）联系起来，表明颗粒表面积最明显的剂量反应。我们的研究表明，Brunauer、Emmett 和 Teller 开发的表面积测量方法是评估碳质 UFP 对健康造成的影响的一个有价值的参考单位。此外，我们证明了在大约 20 cm2 的滴注剂量下存在颗粒表面积的阈值，低于该阈值在小鼠中无法检测到急性促炎反应。
Increased levels of particulate air pollution are associated with increased respiratory and cardiovascular mortality and morbidity. Some epidemiologic and toxicologic research suggests ultrafine particles (UFPs) (< 100 nm) to be more harmful per unit mass than larger particles. Our study was aimed at a quantitative comparison of acute adverse effects of different types of carbonaceous UFPs at a dose range that causes a moderate inflammatory response in lungs. We used six different particle types (primary particle size 10–50 nm, specific surface area 30–800 m2/g, and organic content 1–20%): PrintexG, Printex90, flame soot particles with different organic content (SootL, SootH), spark-generated ultrafine carbon particles (ufCP), and the reference diesel exhaust particles (DEP) SRM1650a. Mice were instilled with 5, 20, and 50 μg of each particle type, and bronchoalveolar lavage was analyzed 24 hr after instillation for inflammatory cells and the level of proinflammatory cytokines. At respective mass-doses, particle-caused detrimental effects ranked in the following order: ufCP > SootL ≥ SootH > Printex90 > PrintexG > DEP. Relating the inflammatory effects to the particle characteristics—organic content, primary particle size, or specific surface area—demonstrates the most obvious dose response for particle surface area. Our study suggests that the surface area measurement developed by Brunauer, Emmett, and Teller is a valuable reference unit for the assessment of causative health effects for carbonaceous UFPs. Additionally, we demonstrated the existence of a threshold for the particle surface area at an instilled dose of approximately 20 cm2, below which no acute proinflammatory responses could be detected in mice.
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影响因子：
3.3
作者：
Brown, RH;Walters, DM;Mitzner, W
通讯作者：
Mitzner, W
影响因子：
11.4
作者：
Hughes, LS;Cass, GR;Olmez, I
通讯作者：
Olmez, I
影响因子：
2.1
作者：
Dick, CAJ;Brown, DM;Stone, V
通讯作者：
Stone, V
影响因子：
2.1
作者：
Oberdörster, G
通讯作者：
Oberdörster, G
影响因子：
2.1
作者：
Daigle, CC;Chalupa, DC;Frampton, MW
通讯作者：
Frampton, MW